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验证数据展示

  • WB analysis using 28219-1-AP (same clone as 28219-1-PBS)

    WB analysis using 28219-1-AP (same clone as 28219-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 28219-1-AP (PCSK1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28219-1-PBS in a different storage buffer formulation.

  • IHC staining of human pancreas using 28219-1-AP (same clone as 28219-1-PBS)

    IHC staining of human pancreas using 28219-1-AP (same clone as 28219-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 28219-1-AP (PCSK1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28219-1-PBS in a different storage buffer formulation.

  • IHC staining of human pancreas using 28219-1-AP (same clone as 28219-1-PBS)

    IHC staining of human pancreas using 28219-1-AP (same clone as 28219-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 28219-1-AP (PCSK1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28219-1-PBS in a different storage buffer formulation.

PCSK1 Polyclonal antibody, PBS Only

PCSK1 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 28219-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

28219-1-PBS targets PCSK1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag28203

Product name: Recombinant human PCSK1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 406-585 aa of BC130295

Sequence: DMQHLVVWTSEYDPLANNPGWKKNGAGLMVNSRFGFGLLNAKALVDLADPRTWRSVPEKKECVVKDNDFEPRALKANGEVIIEIPTRACEGQENAIKSLEHVQFEATIEYSRRGDLHVTLTSAAGTSTVLLAERERDTSPNGFKNWDFMSVHTWGENPIGTWTLRITDMSGRIQNEGRIV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 proprotein convertase subtilisin/kexin type 1
别名
计算分子量 753 aa, 84 kDa
观测分子量66 kDa
GenBank蛋白编号BC130295
基因名称 PCSK1
Gene ID (NCBI) 5122
RRIDAB_2881093
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP29120
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PC1/3, encoded by the gene PCSK1, is synthesized in the endoplasmic reticulum (ER) as an inactive 94 kDa zymogen, where it is rapidly cleaved into a partially active 87-kDa precursor. When this protein reaches the secretory granules, the maturation process continues to generate a 74 kDa species and ends with further cleavage to a fully active, although enzymatically unstable, 66 kDa protein.

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