验证数据展示
经过测试的应用
| Positive WB detected in | BxPC-3 cells, HeLa cells |
| Positive IHC detected in | human pancreas tissue, mouse pancreas tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
| IHC | See 2 publications below |
产品信息
28219-1-AP targets PCSK1 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | Human, Mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag28203 Product name: Recombinant human PCSK1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 406-585 aa of BC130295 Sequence: DMQHLVVWTSEYDPLANNPGWKKNGAGLMVNSRFGFGLLNAKALVDLADPRTWRSVPEKKECVVKDNDFEPRALKANGEVIIEIPTRACEGQENAIKSLEHVQFEATIEYSRRGDLHVTLTSAAGTSTVLLAERERDTSPNGFKNWDFMSVHTWGENPIGTWTLRITDMSGRIQNEGRIV 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | proprotein convertase subtilisin/kexin type 1 |
| 别名 | BMIQ12, NEC 1, NEC1, Neuroendocrine convertase 1, PC1, PC3, PCSK1, Prohormone convertase 1, Proprotein convertase 1, SPC3 |
| 计算分子量 | 753 aa, 84 kDa |
| 观测分子量 | 66 kDa |
| GenBank蛋白编号 | BC130295 |
| 基因名称 | PCSK1 |
| Gene ID (NCBI) | 5122 |
| RRID | AB_2881093 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P29120 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PC1/3, encoded by the gene PCSK1, is synthesized in the endoplasmic reticulum (ER) as an inactive 94 kDa zymogen, where it is rapidly cleaved into a partially active 87-kDa precursor. When this protein reaches the secretory granules, the maturation process continues to generate a 74 kDa species and ends with further cleavage to a fully active, although enzymatically unstable, 66 kDa protein.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for PCSK1 antibody 28219-1-AP | Download protocol |
| WB protocol for PCSK1 antibody 28219-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Nutr Food Res The Effect of Coenzyme Q10 Supplementation on Bile Acid Metabolism: Insights from Network Pharmacology, Molecular Docking, and Experimental Validation | ||
bioRxiv Optimization of protocols for immunohistochemical assessment of enteric nervous system in formalin fixed human tissue | ||
Rapid Commun Mass Spectrom Data-independent acquisition combined with liquid chromatography mass spectrometry technique to detect prognostic protein markers in type I gastric neuroendocrine neoplasm |
