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验证数据展示

  • IF Staining of mouse brain using CL488-14705

    IF Staining of mouse brain using CL488-14705

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 488 PCP4 antibody (CL488-14705) at dilution of 1:200.

  • IF Staining of mouse brain using CL488-14705

    IF Staining of mouse brain using CL488-14705

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 488 PCP4 antibody (CL488-14705) at dilution of 1:200.

  • IF Staining of mouse brain using CL488-14705

    IF Staining of mouse brain using CL488-14705

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 488 PCP4 antibody (CL488-14705) at dilution of 1:200, ADCY3 antibody (19492-1-AP, red).

CoraLite® Plus 488-conjugated PCP4 Polyclonal antibody

PCP4 Polyclonal Antibody for IF-P
Cat No. CL488-14705

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF-P

别名

Brain specific antigen PCP 4, PCP4, PEP 19, PEP19, Purkinje cell protein 4

缓冲液配方:  PBS, Proclin 300, BSA, Glycerol
PBS, Proclin 300, BSA, Glycerol
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IF-P detected inmouse brain tissue
Planning an IHC experiment? We recommend our IHCeasy PCP4 Ready-To-Use IHC Kit. PCP4 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 14705-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-14705 targets PCP4 in IF-P applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF-P Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag6388

Product name: Recombinant human PCP4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-62 aa of BC013791

Sequence: MSERQGAGATNGKDKTSGENDGQKKVQEEFDIDMDAPETERAAVAIQSQFRKFQKKKAGSQS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 Purkinje cell protein 4
别名 Brain specific antigen PCP 4, PCP4, PEP 19, PEP19, Purkinje cell protein 4
计算分子量 7 kDa
观测分子量 8 kDa
GenBank蛋白编号BC013791
基因名称 PCP4
Gene ID (NCBI) 5121
RRIDAB_2934408
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP48539
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PCP4, also named as PEP19, belongs to a family of proteins involved in calcium transduction signals and binds calmodulin via an IQ motif, in a calcium independent manner. It is mainly expressed in ectoderm and neuroectoderm comprising neural crest derived cells.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 PCP4 antibody CL488-14705Download protocol
Standard Protocols
Click here to view our Standard Protocols
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