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验证数据展示

  • WB analysis using 60172-1-Ig (same clone as 60172-1-PBS)

    WB analysis using 60172-1-Ig (same clone as 60172-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60172-1-Ig (PCMT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60172-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 60172-1-Ig (same clone as 60172-1-PBS)

    IF Staining of HeLa using 60172-1-Ig (same clone as 60172-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using PCMT1 antibody (60172-1-Ig, Clone: 3C8G8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60172-1-PBS in a different storage buffer formulation.

PCMT1 Monoclonal antibody, PBS Only

PCMT1 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 60172-1-PBS

产品说明书

CloneNo. 3C8G8

宿主/亚型

Mouse / IgG1

种属反应性

human, pig

应用

WB, IF/ICC, Indirect ELISA

别名

3C8G8

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

60172-1-PBS targets PCMT1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, pig samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, pig
免疫原

CatNo: Ag6277

Product name: Recombinant human PCMT1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-227 aa of BC008748

Sequence: MAWKSGGASHSELIHNLRKNGIIKTDKVFEVMLATDRSHYAKCNPYMDSPQSIGFQATISAPHMHAYALELLFDQLHEGAKALDVGSGSGILTACFARMVGCTGKVIGIDHIKELVDDSINNVRKDDPTLLSSGRVQLVVGDGRMGYAEEAPYDAIHVGAAAPVVPQALIDQLKPGGRLILPVGPAGGNQMLEQYDKLQDGSIKMKPLMGVIYVPLTDKEKQWSRWK

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 protein-L-isoaspartate (D-aspartate) O-methyltransferase
别名 3C8G8
计算分子量 25 kDa
观测分子量 25-27 kDa
GenBank蛋白编号BC008748
基因名称 PCMT1
Gene ID (NCBI) 5110
RRIDAB_10695773
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP22061
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The PCMT1 gene encodes the protein repair enzyme protein-L-isoaspartate (D-aspartate) O-methyltransferase, which is known to protect certain neural cells against Bax-induced apoptosis.

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