验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, Raji cells, pig brain tissue, HEK-293 cells, HepG2 cells, K-562 cells |
| Positive IF/ICC detected in | HeLa cells |
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:10000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
60172-1-Ig targets PCMT1 in WB, IF/ICC, ELISA applications and shows reactivity with human, pig samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 经测试反应性 | human, pig |
| 免疫原 |
CatNo: Ag6277 Product name: Recombinant human PCMT1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-227 aa of BC008748 Sequence: MAWKSGGASHSELIHNLRKNGIIKTDKVFEVMLATDRSHYAKCNPYMDSPQSIGFQATISAPHMHAYALELLFDQLHEGAKALDVGSGSGILTACFARMVGCTGKVIGIDHIKELVDDSINNVRKDDPTLLSSGRVQLVVGDGRMGYAEEAPYDAIHVGAAAPVVPQALIDQLKPGGRLILPVGPAGGNQMLEQYDKLQDGSIKMKPLMGVIYVPLTDKEKQWSRWK 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | protein-L-isoaspartate (D-aspartate) O-methyltransferase |
| 别名 | 3C8G8 |
| 计算分子量 | 25 kDa |
| 观测分子量 | 25-27 kDa |
| GenBank蛋白编号 | BC008748 |
| 基因名称 | PCMT1 |
| Gene ID (NCBI) | 5110 |
| RRID | AB_10695773 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P22061 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The PCMT1 gene encodes the protein repair enzyme protein-L-isoaspartate (D-aspartate) O-methyltransferase, which is known to protect certain neural cells against Bax-induced apoptosis.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for PCMT1 antibody 60172-1-Ig | Download protocol |
| WB protocol for PCMT1 antibody 60172-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
