验证数据展示
经过测试的应用
| Positive IF/ICC detected in | MCF-7 cells |
| Positive FC (Intra) detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-67676 targets PCK2 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat, pig, rabbit samples.
| 经测试应用 | IF/ICC, FC (Intra) Application Description |
| 经测试反应性 | human, mouse, rat, pig, rabbit |
| 免疫原 |
CatNo: Ag7021 Product name: Recombinant human PCK2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 292-640 aa of BC001454 Sequence: AGKKRYVAAAFPSACGKTNLAMMRPALPGWKVECVGDDIAWMRFDSEGRLRAINPENGFFGVAPGTSATTNPNAMATIQSNTIFTNVAETSDGGVYWEGIDQPLPPGVTVTSWLGKPWKPGDKEPCAHPNSRFCAPARQCPIMDPAWEAPEGVPIDAIIFGGRRPKGVPLVYEAFNWRHGVFVGSAMRSESTAAAEHKGKIIMHDPFAMRPFFGYNFGHYLEHWLSMEGRKGAQLPRIFHVNWFRRDEAGHFLWPGFGENARVLDWICRRLEGEDSARETPIGLVPKEGALDLSGLRAIDTTQLFSLPKDFWEQEVRDIRSYLTEQVNQDLPKEVLAELEALERRVHKM 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | phosphoenolpyruvate carboxykinase 2 (mitochondrial) |
| 别名 | 1B3F1, EC:4.1.1.32, mtPCK2, PEPCK2, PEPCK-M |
| 计算分子量 | 71 kDa |
| 观测分子量 | 68 kDa |
| GenBank蛋白编号 | BC001454 |
| 基因名称 | PCK2 |
| Gene ID (NCBI) | 5106 |
| RRID | AB_3084372 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | Q16822 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PCK2(phosphoenolpyruvate carboxykinase [GTP], mitochondrial) is also named as PEPCK2, PEPCK-M and belongs to the phosphoenolpyruvate carboxykinase [GTP] family. It catalyses the irreversible conversion of oxaloacetate to phosphoenolpyruvate, an important initial step in hepatic gluconeogenesis. Defects in PCK2 are the cause of mitochondrial phosphoenolpyruvate carboxykinase deficiency (M-PEPCKD). It has 2 isoforms produced by alternative splicing with the molecular weight of 71 kDa and 48 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 488 PCK2 antibody CL488-67676 | Download protocol |
| IF protocol for CL Plus 488 PCK2 antibody CL488-67676 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
