• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • FC experiment of HeLa using CL488-67676

    FC experiment of HeLa using CL488-67676

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human PCK2 (CL488-67676, Clone:1B3F1) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of MCF-7 using CL488-67676

    IF Staining of MCF-7 using CL488-67676

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CoraLite® Plus 488 PCK2 antibody (CL488-67676, Clone: 1B3F1 ) at dilution of 1:200, CL594-phalloidin (red).

CoraLite® Plus 488-conjugated PCK2 Monoclonal antibody

PCK2 Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-67676

产品说明书

CloneNo. 1B3F1

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig, rabbit

应用

IF/ICC, FC (Intra)

别名

1B3F1, EC:4.1.1.32, mtPCK2, PEPCK2, PEPCK-M

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive IF/ICC detected inMCF-7 cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy PCK2 Ready-To-Use IHC Kit. PCK2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67676-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67676 targets PCK2 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat, pig, rabbit
免疫原 PCK2 fusion protein Ag7021 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 phosphoenolpyruvate carboxykinase 2 (mitochondrial)
别名 1B3F1, EC:4.1.1.32, mtPCK2, PEPCK2, PEPCK-M
计算分子量 71 kDa
观测分子量68 kDa
GenBank蛋白编号BC001454
基因名称 PCK2
Gene ID (NCBI) 5106
RRIDAB_3084372
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ16822
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PCK2(phosphoenolpyruvate carboxykinase [GTP], mitochondrial) is also named as PEPCK2, PEPCK-M and belongs to the phosphoenolpyruvate carboxykinase [GTP] family. It catalyses the irreversible conversion of oxaloacetate to phosphoenolpyruvate, an important initial step in hepatic gluconeogenesis. Defects in PCK2 are the cause of mitochondrial phosphoenolpyruvate carboxykinase deficiency (M-PEPCKD). It has 2 isoforms produced by alternative splicing with the molecular weight of 71 kDa and 48 kDa.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 PCK2 antibody CL488-67676Download protocol
FC protocol for CL Plus 488 PCK2 antibody CL488-67676Download protocol
Standard Protocols
Click here to view our Standard Protocols