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CoraLite® Plus 488-conjugated PCK2 Monoclonal antibody

PCK2 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig, rabbit

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1B3F1

Cat No : CL488-67676

Print datasheet

Synonyms

PCK2, PEPCK, PEPCK M, PEPCK2

验证数据展示

  • FC experiment of HeLa using CL488-67676

    FC experiment of HeLa using CL488-67676

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human PCK2 (CL488-67676, Clone:1B3F1) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy PCK2 Ready-To-Use IHC Kit. PCK2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67676-1-Ig

推荐稀释比

ApplicationDilution
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67676 targets PCK2 in FC (Intra) applications and shows reactivity with Human, mouse, rat, pig, rabbit samples.

Tested Applications FC (Intra)
Tested Reactivity Human, mouse, rat, pig, rabbit
Immunogen PCK2 fusion protein Ag7021 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name phosphoenolpyruvate carboxykinase 2 (mitochondrial)
Synonyms PCK2, PEPCK, PEPCK M, PEPCK2
Calculated Molecular Weight 71 kDa
Observed Molecular Weight68 kDa
GenBank Accession NumberBC001454
Gene Symbol PCK2
Gene ID (NCBI) 5106
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ16822
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PCK2(phosphoenolpyruvate carboxykinase [GTP], mitochondrial) is also named as PEPCK2, PEPCK-M and belongs to the phosphoenolpyruvate carboxykinase [GTP] family. It catalyses the irreversible conversion of oxaloacetate to phosphoenolpyruvate, an important initial step in hepatic gluconeogenesis. Defects in PCK2 are the cause of mitochondrial phosphoenolpyruvate carboxykinase deficiency (M-PEPCKD). It has 2 isoforms produced by alternative splicing with the molecular weight of 71 kDa and 48 kDa.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 PCK2 antibody CL488-67676Download protocol
Standard Protocols
Click here to view our Standard Protocols