验证数据展示
经过测试的应用
| Positive WB detected in | mouse liver tissue |
| Positive IHC detected in | human kidney tissue, human liver tissue, human small intestine tissue, mouse small intestine tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy PCDH24 Ready-To-Use IHC Kit. PCDH24 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:2000-1:8000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
| IHC | See 1 publications below |
产品信息
27103-1-AP targets PCDH24 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human |
| 免疫原 | PCDH24 fusion protein Ag25840 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | protocadherin 24 |
| 别名 | Protocadherin-24, PC-LKC, PCLKC, PC LKC, CDHR2 |
| 计算分子量 | 1310 aa, 142 kDa |
| 观测分子量 | 140-150 kDa |
| GenBank蛋白编号 | BC130282 |
| 基因名称 | PCDH24 |
| Gene ID (NCBI) | 54825 |
| RRID | AB_2880757 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9BYE9 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Protocadherin-24 (PCDH24, also known as CDHR2 and protocadherin LKC) was originally identified as a novel member of the human non-classical cadherin family. PCDH24 may play a role in cell-cell adhesion and contact inhibition in epithelial cells. PCDH24 expression is markedly reduced in cancers derived from liver and colon tissues, indicating that PCDH24 may play a role as a tumor suppressor protein (PMID: 23115611). The calculated molecular weight of PCDH24 is 142 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PCDH24 antibody 27103-1-AP | Download protocol |
| IHC protocol for PCDH24 antibody 27103-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
