验证数据展示
经过测试的应用
| Positive WB detected in | mouse heart tissue, rat heart tissue |
| Positive IHC detected in | human kidney tissue, human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human kidney tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11139-1-AP targets PCCB in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF-P, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag1608 Product name: Recombinant human PCCB protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 240-539 aa of BC013768 Sequence: TQEELGGAKTHTTMSGVAHRAFENDVDALCNLRDFFNYLPLSSQDPAPVRECHDPSDRLVPELDTIVPLESTKAYNMVDIIHSVVDEREFFEIMPNYAKNIIVGFARMNGRTVGIVGNQPKVASGCLDINSSVKGARFVRFCDAFNIPLITFVDVPGFLPGTAQEYGGIIRHGAKLLYAFAEATVPKVTVITRKAYGGAYDVMSSKHLCGDTNYAWPTAEIAVMGAKGAVEIIFKGHENVEAAQAEYIEKFANPFPAAVRGFVDDIIQPSSTRARICCDLDVLASKKVQRPWRKHANIPL 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | propionyl Coenzyme A carboxylase, beta polypeptide |
| 别名 | Propionyl-CoA carboxylase beta chain, mitochondrial, Propanoyl-CoA:carbon dioxide ligase subunit beta, PCCase subunit beta, EC:6.4.1.3 |
| 计算分子量 | 58 kDa |
| 观测分子量 | 58 kDa |
| GenBank蛋白编号 | BC013768 |
| 基因名称 | PCCB |
| Gene ID (NCBI) | 5096 |
| RRID | AB_2877746 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P05166 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for PCCB antibody 11139-1-AP | Download protocol |
| IHC protocol for PCCB antibody 11139-1-AP | Download protocol |
| WB protocol for PCCB antibody 11139-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Eur J Immunol YY1 control of mitochondrial-related genes does not account for regulation of immunoglobulin class switch recombination in mice. | ||
Biochim Biophys Acta Mol Basis Dis ACSS3 regulates the metabolic homeostasis of epithelial cells and alleviates pulmonary fibrosis | ||
Am J Pathol Aging-associated Metabolite Methylmalonic Acid Increases Susceptibility to Pulmonary Fibrosis | ||
Proc Natl Acad Sci U S A Multifunctional biomolecular corona-inspired nanoremediation of antibiotic residues | ||
Cancer Lett PPDPF Promotes Esophageal Squamous Cell Carcinoma Progression by Blocking PCCA Binding to PCCB and Inhibiting Methionine Catabolism |
