验证数据展示
经过测试的应用
| Positive WB detected in | MCF-7 cells, K-562 cells, IMR-32 cells, RAW 264.7 cells, PC-12 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
85180-3-RR targets PCBP3 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag9202 Product name: Recombinant human PCBP3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-313 aa of BC012061 Sequence: MESKVSEGGLNVTLTIRLLMHGKEVGSIIGKKGETVKKMREESGARINISEGNCPERIVTITGPTDAIFKAFAMIAYKFEEDIINSMSNSPATSKPPVTLRLVVPASQCGSLIGKGGSKIKEIRESTGAQVQVAGDMLPNSTERAVTISGTPDAIIQCVKQICVVMLEAYTIQGQYAIPHPDLTKLHQLAMQQTPFPPLGQTNPAFPGEKLPLHSSEEAQNLMGQSSGLDASPPASTHELTIPNDLIGCIIGRQGTKINEIRQMSGAQIKIANATEGSSERQITITGTPANISLAQYLINARLTSEVTGMGTL 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | poly(rC) binding protein 3 |
| 别名 | 242791F11, ALPHA CP3, Alpha-CP3, PCBP3OT, PCBP3-OT1 |
| 计算分子量 | 313 aa, 33 kDa |
| 观测分子量 | 39 kDa |
| GenBank蛋白编号 | BC012061 |
| 基因名称 | PCBP3 |
| Gene ID (NCBI) | 54039 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P57721 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PCBP3 is a member of the KH-domain protein subfamily. Proteins of this subfamily, also referred to as alpha-CPs, bind to RNA with a specificity for C-rich pyrimidine regions. Alpha-CPs play important roles in post-transcriptional activities and have different cellular distributions. PCBP3 is broadly expressed in various tissues, including the testis and endometrium. It has been associated with diseases such as dementia.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PCBP3 antibody 85180-3-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
