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Pyruvate Carboxylase Monoclonal antibody

Pyruvate Carboxylase Monoclonal Antibody for WB, IHC, ELISA
Cat No. 66615-1-Ig

产品说明书

CloneNo. 4F5D1

宿主/亚型

Mouse / IgG2a

种属反应性

Human, Rat , mouse and More (1)

应用

WB, IHC, ELISA and More (1)

PC, PCB, pyruvate carboxykinase, pyruvate carboxylase, Pyruvic carboxylase

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, HSC-T6 cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy PC Ready-To-Use IHC Kit. PC primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66615-1-Ig targets Pyruvate Carboxylase in WB, IHC, RIP, ELISA applications and shows reactivity with Human, Rat , mouse samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, RIP
经测试反应性 Human, Rat , mouse
文献引用反应性human, mouse
免疫原 Pyruvate Carboxylase fusion protein Ag8846 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 pyruvate carboxylase
别名 PC, PCB, pyruvate carboxykinase, pyruvate carboxylase, Pyruvic carboxylase
计算分子量 1178 aa, 130 kDa
观测分子量 130 kDa
GenBank蛋白编号BC011617
基因名称 Pyruvate Carboxylase
Gene ID (NCBI) 5091
RRIDAB_2881975
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP11498
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for Pyruvate Carboxylase antibody 66615-1-IgDownload protocol
IHC protocol for Pyruvate Carboxylase antibody 66615-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Cell Metab

Acetate enables metabolic fitness and cognitive performance during sleep disruption

Authors - Qinqin He
humanWB,RIP

Front Oncol

piR-hsa-211106 Inhibits the Progression of Lung Adenocarcinoma Through Pyruvate Carboxylase and Enhances Chemotherapy Sensitivity.

Authors - Yongmei Liu
humanWB

Elife

Mitochondrial redox adaptations enable alternative aspartate synthesis in SDH-deficient cells

Authors - Madeleine L Hart
  • KO Validated
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