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验证数据展示

  • WB analysis using 11289-1-AP (same clone as 11289-1-PBS)

    WB analysis using 11289-1-AP (same clone as 11289-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11289-1-AP (PARP3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11289-1-PBS in a different storage buffer formulation.

  • IHC staining of human pancreas cancer using 11289-1-AP (same clone as 11289-1-PBS)

    IHC staining of human pancreas cancer using 11289-1-AP (same clone as 11289-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 11289-1-AP (PARP3 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11289-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 11289-1-AP (same clone as 11289-1-PBS)

    IHC staining of human breast cancer using 11289-1-AP (same clone as 11289-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11289-1-AP (PARP3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11289-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 11289-1-AP (same clone as 11289-1-PBS)

    IF Staining of HeLa using 11289-1-AP (same clone as 11289-1-PBS)

    Immunofluorescent analysis of Hela cells, using PARP3 antibody 11289-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 11289-1-PBS in a different storage buffer formulation.

  • WB analysis of human kidney using 11289-1-AP (same clone as 11289-1-PBS)

    WB analysis of human kidney using 11289-1-AP (same clone as 11289-1-PBS)

    human kidney tissue were subjected to SDS PAGE followed by western blot with 11289-1-AP (PARP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11289-1-PBS in a different storage buffer formulation.

  • IHC staining of human renal cell carcinoma using 11289-1-AP (same clone as 11289-1-PBS)

    IHC staining of human renal cell carcinoma using 11289-1-AP (same clone as 11289-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 11289-1-AP (PARP3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11289-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 11289-1-AP (same clone as 11289-1-PBS)

    IHC staining of human breast cancer using 11289-1-AP (same clone as 11289-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11289-1-AP (PARP3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11289-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

PARP3 Polyclonal antibody, PBS Only

PARP3 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 11289-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

hPARP 3, EC:2.4.2.-, DNA ADP-ribosyltransferase PARP3, ARTD3, ADPRT3

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

11289-1-PBS targets PARP3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag1844

Product name: Recombinant human PARP3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 234-533 aa of BC014260

Sequence: EALEEALKGPTDGGQSLEELSSHFYTVIPHNFGHSQPPPINSPELLQAKKDMLLVLADIELAQALQAVSEQEKTVEEVPHPLDRDYQLLKCQLQLLDSGAPEYKVIQTYLEQTGSNHRCPTLQHIWKVNQEGEEDRFQAHSKLGNRKLLWHGTNMAVVAAILTSGLRIMPHSGGRVGKGIYFASENSKSAGYVIGMKCGAHHVGYMFLGEVALGREHHINTDNPSLKSPPPGFDSVIARGHTEPDPTQDTELELDGQQVVVPQGQPVPCPEFSSSTFSQSEYLIYQESQCRLRYLLEVHL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 poly (ADP-ribose) polymerase family, member 3
别名 hPARP 3, EC:2.4.2.-, DNA ADP-ribosyltransferase PARP3, ARTD3, ADPRT3
计算分子量 60 kDa
观测分子量 60-62 kDa
GenBank蛋白编号BC014260
基因名称 PARP3
Gene ID (NCBI) 10039
RRIDAB_2283392
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y6F1
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PARP3 (Poly(ADP-ribose) polymerase 3), also known as ARTD3, is the third member of the PARP family that catalyze a post-translational modification of proteins to promote, control or adjust numerous cellular events including genome integrity, transcription, differentiation, cell metabolism or cell death (PMID: 31095444). PARP3 is a 60-kDa protein containing an N-terminal WGR (tryptophan-, glycine-, and arginine-rich) domain and a C-terminal catalytic domain. PARP3 has been described to interact with partners belonging to the NHEJ pathway including DNA-PKcs, DNA ligase IV, Ku70 and Ku80 and to accelerate XRCC4/DNA ligase IV-mediated ligation of chromosomal DSB in concert with APLF (PMID: 24598253).

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