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验证数据展示

  • WB analysis using 11289-1-AP

    WB analysis using 11289-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11289-1-AP (PARP3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas cancer using 11289-1-AP

    IHC staining of human pancreas cancer using 11289-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 11289-1-AP (PARP3 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human breast cancer using 11289-1-AP

    IHC staining of human breast cancer using 11289-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11289-1-AP (PARP3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 11289-1-AP

    IF Staining of HeLa using 11289-1-AP

    Immunofluorescent analysis of Hela cells, using PARP3 antibody 11289-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • WB analysis of human kidney using 11289-1-AP

    WB analysis of human kidney using 11289-1-AP

    human kidney tissue were subjected to SDS PAGE followed by western blot with 11289-1-AP (PARP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human renal cell carcinoma using 11289-1-AP

    IHC staining of human renal cell carcinoma using 11289-1-AP

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 11289-1-AP (PARP3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 11289-1-AP

    IHC staining of human breast cancer using 11289-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11289-1-AP (PARP3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Featured Product
  • KD/KO验证

PARP3 Polyclonal antibody

PARP3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 11289-1-AP

产品说明书

引用文献(3)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

hPARP 3, EC:2.4.2.-, DNA ADP-ribosyltransferase PARP3, ARTD3, ADPRT3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse heart tissue, human heart tissue, human kidney tissue, rat heart tissue, mouse kidney tissue
Positive IHC detected inhuman breast cancer tissue, human pancreas cancer tissue, human renal cell carcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHela cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11289-1-AP targets PARP3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 PARP3 fusion protein Ag1844 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 poly (ADP-ribose) polymerase family, member 3
别名 hPARP 3, EC:2.4.2.-, DNA ADP-ribosyltransferase PARP3, ARTD3, ADPRT3
计算分子量 60 kDa
观测分子量 60-62 kDa
GenBank蛋白编号BC014260
基因名称 PARP3
Gene ID (NCBI) 10039
RRIDAB_2283392
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y6F1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PARP3 (Poly(ADP-ribose) polymerase 3), also known as ARTD3, is the third member of the PARP family that catalyze a post-translational modification of proteins to promote, control or adjust numerous cellular events including genome integrity, transcription, differentiation, cell metabolism or cell death (PMID: 31095444). PARP3 is a 60-kDa protein containing an N-terminal WGR (tryptophan-, glycine-, and arginine-rich) domain and a C-terminal catalytic domain. PARP3 has been described to interact with partners belonging to the NHEJ pathway including DNA-PKcs, DNA ligase IV, Ku70 and Ku80 and to accelerate XRCC4/DNA ligase IV-mediated ligation of chromosomal DSB in concert with APLF (PMID: 24598253).

实验方案

Product Specific Protocols
WB protocol for PARP3 antibody 11289-1-APDownload protocol
IHC protocol for PARP3 antibody 11289-1-APDownload protocol
IF protocol for PARP3 antibody 11289-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Mol Cell

Unrestrained poly-ADP-ribosylation provides insights into chromatin regulation and human disease.

Authors - Evgeniia Prokhorova
View Article
WB

EBioMedicine

PARP inhibitors suppress tumours via centrosome error-induced senescence independent of DNA damage response

Authors - Wei Yue
View Article
humanWB

Cell Chem Biol

Allosteric regulation of DNA binding and target residence time drive the cytotoxicity of phthalazinone-based PARP-1 inhibitors

Authors - Moriah R Arnold
  • KD Validated
View Article