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验证数据展示

  • WB analysis using 84039-6-RR (same clone as 84039-6-PBS)

    WB analysis using 84039-6-RR (same clone as 84039-6-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84039-6-RR (PARP14 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84039-6-PBS in a different storage buffer formulation.

  • IHC staining of mouse colon using 84039-6-RR (same clone as 84039-6-PBS)

    IHC staining of mouse colon using 84039-6-RR (same clone as 84039-6-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 84039-6-RR (PARP14 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84039-6-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84039-6-RR

    Affinity and Kinetic Characterization of 84039-6-RR

    Biolayer interferometry (BLl) kinetic assays of 84039-6-RR against Human PARP14 were performed. The affinity constant is 0.685 nM.

PARP14 Recombinant monoclonal antibody, PBS Only

PARP14 Uni-rAb® Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 84039-6-PBS

产品说明书

CloneNo. 241078C11

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, Indirect ELISA

别名

PARP 14, KIAA1268, BAL2, 241078C11

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

84039-6-PBS targets PARP14 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag35007

Product name: Recombinant human PARP14 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1501-1640 aa of NM_017554

Sequence: SRDVMQARDEIEAMIKRVRLAKEQESRADCISEFIEWQYNDNNTSHCFNKMTNLKLEDARREKKKTVDVKINHRHYTVNLNTYTATDTKGHSLSVQRLTKSKVDIPAHWSDMKQQNFCVVELLPSDPEYNTVASKFNQTC

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 poly (ADP-ribose) polymerase family, member 14
别名 PARP 14, KIAA1268, BAL2, 241078C11
计算分子量203kd
观测分子量200 kDa
GenBank蛋白编号NM_017554
基因名称 PARP14
Gene ID (NCBI) 54625
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ460N5
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PARP14 (poly (ADP-ribose) polymerase family, member 14) is a PARP family member that has roles in DNA repair, B cell regulation, and focal adhesion (PMID: 25753673). The largest of all the human PARPs is PARP14. PARP14 has been reported to regulate several different pathways involved in immunity, inflammation, and genome stability (PMID: 37703374).

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