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CoraLite® Plus 488-conjugated PARG Monoclonal antibody

PARG Monoclonal Antibody for FC (Intra), IF/ICC

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

FC (Intra), IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1H10G10

Cat No : CL488-67946

Print datasheet

Synonyms

PARG, PARG99

验证数据展示

  • IF Staining of A431 using CL488-67946

    IF Staining of A431 using CL488-67946

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using CoraLite® Plus 488 PARG antibody (CL488-67946, Clone: 1H10G10 ) at dilution of 1:200, CL594-Phalloidin (red).

  • FC experiment of A431 using CL488-67946

    FC experiment of A431 using CL488-67946

    1X10^6 A431 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human PARG (CL488-67946, Clone:1H10G10) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL488-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF detected inA431 cells
Positive FC detected inA431 cells
For other applications, we recommend the unconjugated version of this antibody, 67946-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67946 targets PARG in IF, FC (Intra) applications and shows reactivity with Human samples.

Tested Applications FC (Intra), IF/ICC
Tested Reactivity Human
Immunogen PARG fusion protein Ag27288 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name poly (ADP-ribose) glycohydrolase
Synonyms PARG, PARG99
Calculated Molecular Weight 111 kDa
Observed Molecular Weight111 kDa
GenBank Accession NumberBC052966
Gene Symbol PARG
Gene ID (NCBI) 8505
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ86W56
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 PARG antibody CL488-67946Download protocol
FC protocol for CL Plus 488 PARG antibody CL488-67946Download protocol
Standard Protocols
Click here to view our Standard Protocols