PARD6B Polyclonal antibody
PARD6B Polyclonal Antibody for FC, IF/ICC, IHC, IP, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
FC, IF/ICC, IHC, IP, WB,ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HEK-293 cells, human placenta tissue, PC-3 cells |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | mouse kidney tissue, human pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HepG2 cells, MCF-7 cells |
| Positive FC detected in | HepG2 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:10-1:100 |
| Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
| IF | See 1 publications below |
产品信息
13996-1-AP targets PARD6B in WB, IP, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | FC, IF/ICC, IHC, IP, WB,ELISA |
| Cited Applications | WB, IF |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse, rat |
| Immunogen | PARD6B fusion protein Ag5083 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | par-6 partitioning defective 6 homolog beta (C. elegans) |
| Synonyms | PAR 6 beta, PAR 6B, PAR6B, PARD6B |
| Calculated Molecular Weight | 41 kDa |
| Observed Molecular Weight | 50-53 kDa |
| GenBank Accession Number | BC060847 |
| Gene Symbol | PARD6B |
| Gene ID (NCBI) | 84612 |
| RRID | AB_2159779 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BYG5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PARD6B (also named as PAR6B) is a member of the PAR6 family. PARD6B is known to play a key role in mammary epithelial cell biology. Several studies have been reported which indicate deregulated PARD6B signaling contributes to malignant epithelial cell phenotypes due predominantly to disrupted polymerization and maintenance of tight junctions (PMID: 22957302). The approximately 4.8-kb long PARD6B mRNA was predominantly detected in both adult and foetal kidneys, while much weaker but significant signals were observed in the placenta, lung and liver (PMID: 11260256). This antibody detects PARD6B with an apparent molecular weight of 50-53 kDa as has been demonstrated by several researches (PMID: 22496418; 25662318).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PARD6B antibody 13996-1-AP | Download protocol |
| IHC protocol for PARD6B antibody 13996-1-AP | Download protocol |
| IF protocol for PARD6B antibody 13996-1-AP | Download protocol |
| IP protocol for PARD6B antibody 13996-1-AP | Download protocol |
| FC protocol for PARD6B antibody 13996-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
iScience Induced retinal pigment epithelial cells with anti-epithelial-to-mesenchymal transition ability delay retinal degeneration | ||
PLoS One PKCζ and JNK signaling regulate radiation-induced compensatory proliferation in parotid salivary glands. | ||
Theriogenology Influence of Wilms' tumor suppressor gene WT1 on bovine Sertoli cells polarity and tight junctions via non-canonical WNT signaling pathway. |
