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PAPPA Monoclonal antibody

PAPPA Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (1)

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

1G5A7

Cat No : 66962-1-Ig

Print datasheet

Synonyms

ASBABP2, DIPLA1, IGF dependent IGFBP 4 protease, IGFBP 4ase, PAPA, PAPP A, PAPPA, PAPP-A, PAPPA1, Pappalysin 1, Pappalysin-1

验证数据展示

  • WB analysis of human placenta using 66962-1-Ig

    WB analysis of human placenta using 66962-1-Ig

    human placenta tissue were subjected to SDS PAGE followed by western blot with 66962-1-Ig (PAPPA antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • WB analysis of MCF-7 using 66962-1-Ig

    WB analysis of MCF-7 using 66962-1-Ig

    WB result of PAPPA antibody (66962-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PAPPA transfected MCF-7 cells.

  • IHC staining of human placenta using 66962-1-Ig

    IHC staining of human placenta using 66962-1-Ig

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66962-1-Ig (PAPPA antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PAPPA antibody included
  • IHC staining of human placenta using 66962-1-Ig

    IHC staining of human placenta using 66962-1-Ig

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66962-1-Ig (PAPPA antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PAPPA antibody included

经过测试的应用

Positive WB detected inhuman placenta tissue, MCF-7 cells
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy PAPPA Ready-To-Use IHC Kit. PAPPA primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:4000-1:10000
Immunohistochemistry (IHC)IHC : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

66962-1-Ig targets PAPPA in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Applications IHC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity Human
Cited Reactivityhuman
Immunogen PAPPA fusion protein Ag11863 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name pregnancy-associated plasma protein A, pappalysin 1
Synonyms ASBABP2, DIPLA1, IGF dependent IGFBP 4 protease, IGFBP 4ase, PAPA, PAPP A, PAPPA, PAPP-A, PAPPA1, Pappalysin 1, Pappalysin-1
Calculated Molecular Weight 1627 aa, 181 kDa
Observed Molecular Weight 180-220 kDa
GenBank Accession NumberBC078657
Gene Symbol PAPPA
Gene ID (NCBI) 5069
RRIDAB_2882285
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13219
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Pregnancy-associated plasma protein A (PAPPA) is a metalloproteinase secreted by human placenta, and it can interact with IGF-binding proteins (IGFBPs) to regulate the proteolysis and the bioavailability of insulin-like growth factor (IGF). Circulating PAPPA represents a consolidated biomarker in combined first-trimester screening tests, allowing an early prenatal diagnosis of chromosomal abnormalities, preeclampsia, and intrauterine growth restriction risk. PAPPA is highly expressed in placenta and pregnancy serum (PMID: 34974815, 34974815).

实验方案

Product Specific Protocols
WB protocol for PAPPA antibody 66962-1-IgDownload protocol
IHC protocol for PAPPA antibody 66962-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Mol Oncol

Cancer-associated fibroblasts educate normal fibroblasts to facilitate cancer cell spreading and T cell suppression.

Authors - Go Itoh
View Article