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验证数据展示

  • WB analysis of human placenta using 66962-1-Ig (same clone as 66962-1-PBS)

    WB analysis of human placenta using 66962-1-Ig (same clone as 66962-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 66962-1-Ig (PAPPA antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66962-1-PBS in a different storage buffer formulation.

  • WB analysis of MCF-7 using 66962-1-Ig (same clone as 66962-1-PBS)

    WB analysis of MCF-7 using 66962-1-Ig (same clone as 66962-1-PBS)

    WB result of PAPPA antibody (66962-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PAPPA transfected MCF-7 cells. This data was developed using the same antibody clone with 66962-1-PBS in a different storage buffer formulation.

  • IHC staining of human placenta using 66962-1-Ig (same clone as 66962-1-PBS)

    IHC staining of human placenta using 66962-1-Ig (same clone as 66962-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66962-1-Ig (PAPPA antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66962-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PAPPA antibody included
  • IHC staining of human placenta using 66962-1-Ig (same clone as 66962-1-PBS)

    IHC staining of human placenta using 66962-1-Ig (same clone as 66962-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66962-1-Ig (PAPPA antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66962-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PAPPA antibody included
  • Featured Product
  • KD/KO验证

PAPPA Monoclonal antibody, PBS Only

PAPPA Monoclonal Antibody for WB, IHC, ELISA
Cat No. 66962-1-PBS

产品说明书

CloneNo. 1G5A7

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IHC, ELISA

别名

1G5A7, ASBABP2, DIPLA1, EC:3.4.24.79, IGFBP 4ase

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy PAPPA Ready-To-Use IHC Kit. PAPPA primary antibody included.

产品信息

66962-1-PBS targets PAPPA in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag11863

Product name: Recombinant human PAPPA protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1279-1627 aa of BC078657

Sequence: ACEPVDCSIPDHHQVYAASFSCPEGTTFGSQCSFQCRHPAQLKGNNSLLTCMEDGLWSFPEALCELMCLAPPPVPNADLQTARCRENKHKVGSFCKYKCKPGYHVPGSSRKSKKRAFKTQCTQDGSWQEGACVPVTCDPPPPKFHGLYQCTNGFQFNSECRIKCEDSDASQGLGSNVIHCRKDGTWNGSFHVCQEMQGQCSVPNELNSNLKLQCPDGYAIGSECATSCLDHNSESIILPMNVTVRDIPHWLNPTRVERVVCTAGLKWYPHPALIHCVKGCEPFMGDNYCDAINNRAFCNYDGGDCCTSTVKTKKVTPFPMSCDLQGDCACRDPQAQEHSRKDLQGYSHG

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 pregnancy-associated plasma protein A, pappalysin 1
别名 1G5A7, ASBABP2, DIPLA1, EC:3.4.24.79, IGFBP 4ase
计算分子量 1627 aa, 181 kDa
观测分子量 180-220 kDa
GenBank蛋白编号BC078657
基因名称 PAPPA
Gene ID (NCBI) 5069
RRIDAB_2882285
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ13219
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Pregnancy-associated plasma protein A (PAPPA) is a metalloproteinase secreted by human placenta, and it can interact with IGF-binding proteins (IGFBPs) to regulate the proteolysis and the bioavailability of insulin-like growth factor (IGF). Circulating PAPPA represents a consolidated biomarker in combined first-trimester screening tests, allowing an early prenatal diagnosis of chromosomal abnormalities, preeclampsia, and intrauterine growth restriction risk. PAPPA is highly expressed in placenta and pregnancy serum (PMID: 34974815, 34974815).

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