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验证数据展示

  • WB analysis of HeLa using 24284-1-AP (same clone as 24284-1-PBS)

    WB analysis of HeLa using 24284-1-AP (same clone as 24284-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 24284-1-AP (PAPOLG Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24284-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 24284-1-AP (same clone as 24284-1-PBS)

    IP experiment of HeLa using 24284-1-AP (same clone as 24284-1-PBS)

    IP result of anti-PAPOLG (IP:24284-1-AP, 4ug; Detection:24284-1-AP 1:1000) with HeLa cells lysate 3200ug. This data was developed using the same antibody clone with 24284-1-PBS in a different storage buffer formulation.

  • IHC staining of human testis using 24284-1-AP (same clone as 24284-1-PBS)

    IHC staining of human testis using 24284-1-AP (same clone as 24284-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24284-1-AP (PAPOLG Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 24284-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 24284-1-AP (same clone as 24284-1-PBS)

    IF Staining of HepG2 using 24284-1-AP (same clone as 24284-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PAPOLG antibody (24284-1-AP) at dilution of 1:1600 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 24284-1-PBS in a different storage buffer formulation.

PAPOLG Polyclonal antibody, PBS Only

PAPOLG Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 24284-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

24284-1-PBS targets PAPOLG in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag16208

Product name: Recombinant human PAPOLG protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 469-736 aa of BC111701

Sequence: QANNINMLKEGMKIEATHVKKKQLHHYLPAEILQKKKKQSLSDVNRSSGGLQSKRLSLDSSCLDSSRDTDNGTPFNSPASKSDSPSVGETERNSAEPAAVIVEKPLSVPPAQGLSIPVIGAKVDSTVKTVSPPTVCTIPTVVGRNVIPRITTPHNPAQGQPHLNGMSNITKTVTPKRSHSPSIDGTPKRLKDVEKFIRLESTFKDPRTAEERKRKSVDAIGGESMPIPTIDTSRKKRLPSKELPDSSSPVPANNIRVIKNSIRLTLNR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 poly(A) polymerase gamma
别名
计算分子量 736 aa, 83 kDa
观测分子量 83 kDa
GenBank蛋白编号BC111701
基因名称 PAPOLG
Gene ID (NCBI) 64895
RRIDAB_2879471
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BWT3
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PAPOLG, also named as Neo-poly(A) polymerase or Polynucleotide adenylyltransferase gamma, is a 736 amino acid protein, which belongs to the poly(A) polymerase family. PAPOLG is expressed predominantly in testis, and weakly in other tissues. PAPOLG is responsible for the post-transcriptional adenylation of the 3'-terminal of mRNA precursors and several small RNAs including signal recognition particle (SRP) RNA, nuclear 7SK RNA, U2 small nuclear RNA, and ribosomal 5S RNA.

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