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PAPOLG Polyclonal antibody

PAPOLG Polyclonal Antibody for IF/ICC, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, IHC, IP, WB,ELISA

Conjugate

Unconjugated

Cat No : 24284-1-AP

Print datasheet

Synonyms

Neo PAP, Neo poly(A) polymerase, PAP gamma, PAP2, PAPG, PAPOLG, poly(A) polymerase gamma, SRP RNA 3 adenylating enzyme

验证数据展示

  • WB analysis of HeLa using 24284-1-AP

    WB analysis of HeLa using 24284-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 24284-1-AP (PAPOLG Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 24284-1-AP

    IP experiment of HeLa using 24284-1-AP

    IP result of anti-PAPOLG (IP:24284-1-AP, 4ug; Detection:24284-1-AP 1:1000) with HeLa cells lysate 3200ug.

  • IHC staining of human testis using 24284-1-AP

    IHC staining of human testis using 24284-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24284-1-AP (PAPOLG Antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HeLa using 24284-1-AP

    IF Staining of HeLa using 24284-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 24284-1-AP (PAPOLG antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells, MCF-7 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 24284-1-AP is PAPOLG Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, IHC, IP, WB,ELISA
Tested Reactivity human
Immunogen PAPOLG fusion protein Ag16208 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name poly(A) polymerase gamma
Synonyms Neo PAP, Neo poly(A) polymerase, PAP gamma, PAP2, PAPG, PAPOLG, poly(A) polymerase gamma, SRP RNA 3 adenylating enzyme
Calculated Molecular Weight 736 aa, 83 kDa
Observed Molecular Weight 83 kDa
GenBank Accession NumberBC111701
Gene Symbol PAPOLG
Gene ID (NCBI) 64895
RRIDAB_2879471
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BWT3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PAPOLG, also named as Neo-poly(A) polymerase or Polynucleotide adenylyltransferase gamma, is a 736 amino acid protein, which belongs to the poly(A) polymerase family. PAPOLG is expressed predominantly in testis, and weakly in other tissues. PAPOLG is responsible for the post-transcriptional adenylation of the 3'-terminal of mRNA precursors and several small RNAs including signal recognition particle (SRP) RNA, nuclear 7SK RNA, U2 small nuclear RNA, and ribosomal 5S RNA.

实验方案

Product Specific Protocols
WB protocol for PAPOLG antibody 24284-1-APDownload protocol
IHC protocol for PAPOLG antibody 24284-1-APDownload protocol
IF protocol for PAPOLG antibody 24284-1-APDownload protocol
IP protocol for PAPOLG antibody 24284-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols