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验证数据展示

  • WB analysis of HeLa using 24284-1-AP

    WB analysis of HeLa using 24284-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 24284-1-AP (PAPOLG Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 24284-1-AP

    IP experiment of HeLa using 24284-1-AP

    IP result of anti-PAPOLG (IP:24284-1-AP, 4ug; Detection:24284-1-AP 1:1000) with HeLa cells lysate 3200ug.

  • IHC staining of human testis using 24284-1-AP

    IHC staining of human testis using 24284-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24284-1-AP (PAPOLG Antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HepG2 using 24284-1-AP

    IF Staining of HepG2 using 24284-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PAPOLG antibody (24284-1-AP) at dilution of 1:1600 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

PAPOLG Polyclonal antibody

PAPOLG Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 24284-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, IP, ELISA

别名

PAP gamma, Neo-poly(A) polymerase, Neo-PAP, NeoPAP, Neo PAP

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, MCF-7 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:800-1:3200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24284-1-AP targets PAPOLG in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
经测试反应性 human
免疫原 PAPOLG fusion protein Ag16208 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 poly(A) polymerase gamma
别名 PAP gamma, Neo-poly(A) polymerase, Neo-PAP, NeoPAP, Neo PAP
计算分子量 736 aa, 83 kDa
观测分子量 83 kDa
GenBank蛋白编号BC111701
基因名称 PAPOLG
Gene ID (NCBI) 64895
RRIDAB_2879471
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BWT3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PAPOLG, also named as Neo-poly(A) polymerase or Polynucleotide adenylyltransferase gamma, is a 736 amino acid protein, which belongs to the poly(A) polymerase family. PAPOLG is expressed predominantly in testis, and weakly in other tissues. PAPOLG is responsible for the post-transcriptional adenylation of the 3'-terminal of mRNA precursors and several small RNAs including signal recognition particle (SRP) RNA, nuclear 7SK RNA, U2 small nuclear RNA, and ribosomal 5S RNA.

实验方案

Product Specific Protocols
WB protocol for PAPOLG antibody 24284-1-APDownload protocol
IHC protocol for PAPOLG antibody 24284-1-APDownload protocol
IF protocol for PAPOLG antibody 24284-1-APDownload protocol
IP protocol for PAPOLG antibody 24284-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols