验证数据展示
产品信息
34287-1-PBS targets PAPOLA in WB, Indirect ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, Indirect ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag41259 Product name: Recombinant human PAPOLA protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 560-745 aa of BC036014 Sequence: APAVTAASVTNIQATEVSVPQVNSSESSGGTSSESIPQTATQPAISPPPKPTVSRVVSSTRLVNPPPRSSGNAATSGNAATKIPTPIVGVKRTSSPHKEESPKKTKTEEDETSEDANCLALSGHDKTEAKEQLDTETSTTQSETIQTAASLLASQKTSSTDLSDIPALPANPIPVIKNSIKLRLNR 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | poly(A) polymerase alpha |
| 别名 | EC:2.7.7.19, PAP, PAP-alpha, Poly(A) polymerase alpha, Polynucleotide adenylyltransferase alpha |
| 计算分子量 | 82 kDa |
| 观测分子量 | 90 kDa |
| GenBank蛋白编号 | BC036014 |
| 基因名称 | PAPOLA |
| Gene ID (NCBI) | 10914 |
| RRID | AB_3743186 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | P51003 |
| 储存缓冲液 | PBS only, pH 7.3. |
| 储存条件 | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
背景介绍
Poly(A) polymerase α (PAPOLA) is the most-studied mammalian poly(A) polymerase. Silencing PAPOLA in MCF-7 and MDA-MB-231 breast cancer cells reduced proliferation and anchorage-independent growth by decreasing steady-state cyclin D1 (CCND1) mRNA and protein levels. PAPOLA is a possible candidate target for the control of tumor growth that is mostly relevant to triple-negative tumors, a group characterized by PAPOLA overexpression and lack of alternative targeted therapies (PMID: 33712453). PAPOLA contains various forms of modifications such as acetylation, phosphorylation, and poly-sulfurization.
