验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, U-251 cells, U-87 MG cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
34287-1-AP targets PAPOLA in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag41259 Product name: Recombinant human PAPOLA protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 560-745 aa of BC036014 Sequence: APAVTAASVTNIQATEVSVPQVNSSESSGGTSSESIPQTATQPAISPPPKPTVSRVVSSTRLVNPPPRSSGNAATSGNAATKIPTPIVGVKRTSSPHKEESPKKTKTEEDETSEDANCLALSGHDKTEAKEQLDTETSTTQSETIQTAASLLASQKTSSTDLSDIPALPANPIPVIKNSIKLRLNR 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | poly(A) polymerase alpha |
| 别名 | EC:2.7.7.19, PAP, PAP-alpha, Poly(A) polymerase alpha, Polynucleotide adenylyltransferase alpha |
| 计算分子量 | 82 kDa |
| 观测分子量 | 90 kDa |
| GenBank蛋白编号 | BC036014 |
| 基因名称 | PAPOLA |
| Gene ID (NCBI) | 10914 |
| RRID | AB_3743186 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | P51003 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Poly(A) polymerase α (PAPOLA) is the most-studied mammalian poly(A) polymerase. Silencing PAPOLA in MCF-7 and MDA-MB-231 breast cancer cells reduced proliferation and anchorage-independent growth by decreasing steady-state cyclin D1 (CCND1) mRNA and protein levels. PAPOLA is a possible candidate target for the control of tumor growth that is mostly relevant to triple-negative tumors, a group characterized by PAPOLA overexpression and lack of alternative targeted therapies (PMID: 33712453). PAPOLA contains various forms of modifications such as acetylation, phosphorylation, and poly-sulfurization.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PAPOLA antibody 34287-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
