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验证数据展示

  • FC experiment of Caco-2 using 98696-1-RR

    FC experiment of Caco-2 using 98696-1-RR

    1x10^6 Caco-2 cells were surface stained with 0.25 ug Anti-Human PANX2 Rabbit RecAb (98696-2-RR, Clone: 251544A12) or 0.25 ug Isotype Control, and APC-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

  • IHC staining of Human brain cancer（Astrocitoma） using 98696-1-RR

    IHC staining of Human brain cancer（Astrocitoma） using 98696-1-RR

    Immunohistochemical analysis of paraffin-embedded Human brain cancer(Astrocitoma) slide using 98696-1-RR (PANX2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of Human brain cancer（Astrocitoma） using 98696-1-RR

    IHC staining of Human brain cancer（Astrocitoma） using 98696-1-RR

    Immunohistochemical analysis of paraffin-embedded Human brain cancer(Astrocitoma) slide using 98696-1-RR (PANX2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • FC experiment of Caco-2 using 98696-1-RR

    FC experiment of Caco-2 using 98696-1-RR

    1x10^6 Caco-2 cells were surface stained with 0.25 ug Anti-Human PANX2 Rabbit RecAb (98696-2-RR, Clone: 251544A12) (red) or 0.25 ug Isotype Control (blue), and APC-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

Anti-Human PANX2 Rabbit Recombinant Antibody

PANX2 Uni-rAb® Recombinant Antibody for IHC, FC
Cat No. 98696-1-RR

产品说明书

CloneNo. 251544B5

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, FC

别名

hPANX2, pannexin 2, PX2

缓冲液配方:  PBS, Azide
PBS, Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IHC detected inHuman brain cancer (Astrocitoma)
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inCaco-2 cells

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in a 100 µl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98696-1-RR targets PANX2 in IHC, FC applications and shows reactivity with human samples.

经测试应用 IHC, FC Application Description
经测试反应性 human
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 pannexin 2
别名 hPANX2, pannexin 2, PX2
计算分子量 677 aa, 74 kDa
GenBank蛋白编号BC101023
基因名称 PANX2
Gene ID (NCBI) 56666
RRIDAB_3746281
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ96RD6
储存缓冲液 PBS with 0.09% sodium azide, pH 7.3.
储存条件Store at 2 - 8°C. Stable for one year after shipment.

背景介绍

Pannexins are glycoproteins that form functional single-membrane channels, which mediate ATP release in response to a variety of physiological and pathological stimuli (PMID: 33913372). The pannexin family consists of three members, namely PANX1, PANX2, and PANX3 (PMID: 22305965). PANX2 was initially predicted to be expressed selectively in the central nervous system, but was later shown to have broad expression across multiple tissues, including skin, skeletal muscle, eye, and colon (PMID: 15028292; 25505382; 25773474). PANX2 is involved in pathophysiological processes of neurological and cancer diseases (PMID: 19749789; 33913372; 39389335).

实验方案

Product Specific Protocols
FC protocol for PANX2 antibody 98696-1-RRDownload protocol
IHC protocol for PANX2 antibody 98696-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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