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验证数据展示

  • WB analysis using 25655-1-AP (same clone as 25655-1-PBS)

    WB analysis using 25655-1-AP (same clone as 25655-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 25655-1-AP (PAN3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25655-1-PBS in a different storage buffer formulation.

  • IHC staining of human ovary cancer using 25655-1-AP (same clone as 25655-1-PBS)

    IHC staining of human ovary cancer using 25655-1-AP (same clone as 25655-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 25655-1-AP (PAN3 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25655-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PAN3 antibody included

PAN3 Polyclonal antibody, PBS Only

PAN3 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 25655-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, Indirect ELISA

别名

hPan3, PAB1P-dependent poly(A)-specific ribonuclease, PAN deadenylation complex subunit 3, PAN2-PAN3 deadenylation complex subunit PAN3, Poly(A)-nuclease deadenylation complex subunit 3

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy PAN3 Ready-To-Use IHC Kit. PAN3 primary antibody included.

产品信息

25655-1-PBS targets PAN3 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag22559

Product name: Recombinant human PAN3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 512-687 aa of BC128180

Sequence: KAMELVTINYSSDLKNLILYLLTDQNRMRSVNDIMPMIGARFYTQLDAAQMRNDVIEEDLAKEVQNGRLFRLLAKLGTINERPEFQKDPTWSETGDRYLLKLFRDHLFHQVTEAGAPWIDLSHIISCLNKLDAGVPEKISLISRDEKSVLVVTYSDLKRCFENTFQELIAAANGQL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 PAN3 poly(A) specific ribonuclease subunit homolog (S. cerevisiae)
别名 hPan3, PAB1P-dependent poly(A)-specific ribonuclease, PAN deadenylation complex subunit 3, PAN2-PAN3 deadenylation complex subunit PAN3, Poly(A)-nuclease deadenylation complex subunit 3
计算分子量 887 aa, 96 kDa
观测分子量 90-100kDa; 65-68 kDa
GenBank蛋白编号BC128180
基因名称 PAN3
Gene ID (NCBI) 255967
RRIDAB_3669491
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ58A45
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Shortening of the poly(A) tail in mRNA, called deadenylation, is the first rate-limiting step in mRNA turnover. PAN3 is the regulatory subunit of a cytoplasmic poly(A) nuclease complex that links the catalytic PAN2 subunit to the poly(A)-binding protein PABP, which stimulates PAN2-dependent deadenylation activity (PMID: 14583602). PAN3 has four isoforms, isoform1: 95 kDa; isoform2: 64 kDa, isoform3: 89 kDa and isoform4: 72 kDa. This antibody recognizes isoform 1/2/3.

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