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PAK7 Monoclonal antibody

PAK7 Monoclonal Antibody for IF/ICC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Pig

Applications

IF/ICC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

1H3B6

Cat No : 66961-1-Ig

Print datasheet

Synonyms

KIAA1264, p21 activated kinase 5, p21 activated kinase 7, PAK 5, PAK 7, PAK5, PAK7

验证数据展示

  • WB analysis of mouse brain using 66961-1-Ig

    WB analysis of mouse brain using 66961-1-Ig

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 66961-1-Ig (PAK7 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of U-251 using 66961-1-Ig

    IF Staining of U-251 using 66961-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed U-251 cells using PAK7 antibody (66961-1-Ig, Clone: 1H3B6 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inmouse brain tissue, pig brain tissue, rat brain tissue
Positive IF detected inU-251 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)IF : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66961-1-Ig targets PAK7 in WB, IF, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

Tested Applications IF/ICC, WB, ELISA
Tested Reactivity Human, Mouse, Rat, Pig
Immunogen PAK7 fusion protein Ag27299 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name p21 protein (Cdc42/Rac)-activated kinase 7
Synonyms KIAA1264, p21 activated kinase 5, p21 activated kinase 7, PAK 5, PAK 7, PAK5, PAK7
Calculated Molecular Weight 719 aa, 81 kDa
Observed Molecular Weight 80 kDa
GenBank Accession NumberBC024179
Gene Symbol PAK7
Gene ID (NCBI) 57144
RRIDAB_2882284
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9P286
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PAK7(p21-activated kinase 7) is also named as KIAA1264, PAK5 and belongs to the STE Ser/Thr protein kinase family. It plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell migration, proliferation or cell survival.

实验方案

Product Specific Protocols
WB protocol for PAK7 antibody 66961-1-IgDownload protocol
IF protocol for PAK7 antibody 66961-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols