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验证数据展示

  • WB analysis of mouse brain using 66961-1-Ig

    WB analysis of mouse brain using 66961-1-Ig

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 66961-1-Ig (PAK7 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of Neuro-2a using 66961-1-Ig

    IF Staining of Neuro-2a using 66961-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed Neuro-2a cells using PAK7 antibody (66961-1-Ig, Clone: 1H3B6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

PAK7 Monoclonal antibody

PAK7 Monoclonal Antibody for WB, IF, ELISA
Cat No. 66961-1-Ig

产品说明书

CloneNo. 1H3B6

宿主/亚型

Mouse / IgG1

种属反应性

Human, Mouse, Rat, Pig

应用

WB, IF, ELISA

别名

KIAA1264, p21 activated kinase 5, p21 activated kinase 7, PAK 5, PAK 7, PAK5, PAK7

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse brain tissue, pig brain tissue, rat brain tissue
Positive IF/ICC detected inNeuro-2a cells, U-251 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66961-1-Ig targets PAK7 in WB, IF, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

经测试应用 WB, IF, ELISA Application Description
经测试反应性 Human, Mouse, Rat, Pig
免疫原

CatNo: Ag27299

Product name: Recombinant human PAK7 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-200 aa of BC024179

Sequence: MFGKKKKKIEISGPSNFEHRVHTGFDPQEQKFTGLPQQWHSLLADTANRPKPMVDPSCITPIQLAPMKTIVRGNKPCKETSINGLLEDFDNISVTRSNSLRKESPPTPDQGASSHGPGHAEENGFITFSQYSSESDTTADYTTEKYREKSLYGDDLDPYYRGSHAAKQNGHVMKMKHGEAYYSEVKPLKSDFARFSADYH

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 p21 protein (Cdc42/Rac)-activated kinase 7
别名 KIAA1264, p21 activated kinase 5, p21 activated kinase 7, PAK 5, PAK 7, PAK5, PAK7
计算分子量 719 aa, 81 kDa
观测分子量 80 kDa
GenBank蛋白编号BC024179
基因名称 PAK7
Gene ID (NCBI) 57144
RRIDAB_2882284
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ9P286
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PAK7(p21-activated kinase 7) is also named as KIAA1264, PAK5 and belongs to the STE Ser/Thr protein kinase family. It plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell migration, proliferation or cell survival.

实验方案

Product Specific Protocols
WB protocol for PAK7 antibody 66961-1-IgDownload protocol
IF protocol for PAK7 antibody 66961-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols