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验证数据展示

  • WB analysis using 13539-1-AP (same clone as 13539-1-PBS)

    WB analysis using 13539-1-AP (same clone as 13539-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 13539-1-AP (PAK6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13539-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse testis using 13539-1-AP (same clone as 13539-1-PBS)

    IP experiment of mouse testis using 13539-1-AP (same clone as 13539-1-PBS)

    IP result of anti-PAK6 (IP:13539-1-AP, 4ug; Detection:13539-1-AP 1:300) with mouse testis tissue lysate 4000 ug. This data was developed using the same antibody clone with 13539-1-PBS in a different storage buffer formulation.

  • IHC staining of human prostate cancer using 13539-1-AP (same clone as 13539-1-PBS)

    IHC staining of human prostate cancer using 13539-1-AP (same clone as 13539-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13539-1-AP (PAK6 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13539-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PAK6 antibody included
  • IHC staining of human prostate cancer using 13539-1-AP (same clone as 13539-1-PBS)

    IHC staining of human prostate cancer using 13539-1-AP (same clone as 13539-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13539-1-AP (PAK6 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13539-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PAK6 antibody included
  • IF Staining of A431 using 13539-1-AP (same clone as 13539-1-PBS)

    IF Staining of A431 using 13539-1-AP (same clone as 13539-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using PAK6 antibody (13539-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 13539-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

PAK6 Polyclonal antibody, PBS Only

PAK6 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 13539-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

PAK5, PAK 6, PAK 5, p21-activated kinase 6, p21 activated kinase 6

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy PAK6 Ready-To-Use IHC Kit. PAK6 primary antibody included.

产品信息

13539-1-PBS targets PAK6 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag4467

Product name: Recombinant human PAK6 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 330-681 aa of BC035596

Sequence: QKSLRTAPATGQLPGRSSPAGSPRTWHAQISTSNLYLPQDPTVAKGALAGEDTGVVTHEQFKAALRMVVDQGDPRLLLDSYVKIGEGSTGIVCLAREKHSGRQVAVKMMDLRKQQRRELLFNEVVIMRDYQHFNVVEMYKSYLVGEELWVLMEFLQGGALTDIVSQVRLNEEQIATVCEAVLQALAYLHAQGVIHRDIKSDSILLTLDGRVKLSDFGFCAQISKDVPKRKSLVGTPYWMAPEVISRSLYATEVDIWSLGIMVIEMVDGEPPYFSDSPVQAMKRLRDSPPPKLKNSHKVSPVLRDFLERMLVRDPQERATAQELLDHPFLLQTGLPECLVPLIQLYRKQTSTC

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 p21 protein (Cdc42/Rac)-activated kinase 6
别名 PAK5, PAK 6, PAK 5, p21-activated kinase 6, p21 activated kinase 6
计算分子量 681 aa, 75 kDa
观测分子量 70-75 kDa
GenBank蛋白编号BC035596
基因名称 PAK6
Gene ID (NCBI) 56924
RRIDAB_2158467
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NQU5
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PAK6(p21-activated kinase 6) is a serine/threonine kinase belonging to the p21-activated kinase (PAK) family. .This protein plays a role in the regulation of gene transcription and may protect cells from apoptosis through phosphorylation of BAD. Northern blot analysis revealed that PAK6 is highly expressed in testis and prostate tissues (PMID:11278661). The immunogen of this antibody is the C-terminal of PAK6. This antibody has weak cross-reaction with other PAK members.

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