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验证数据展示

  • WB analysis using 85646-2-RR (same clone as 85646-2-PBS)

    WB analysis using 85646-2-RR (same clone as 85646-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85646-2-RR (PAK2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85646-2-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 85646-2-RR (same clone as 85646-2-PBS)

    IP experiment of HeLa using 85646-2-RR (same clone as 85646-2-PBS)

    IP result of anti-PAK2 (IP:85646-2-RR, 4ug; Detection:85646-2-RR 1:1000) with HeLa cells lysate 920 ug. This data was developed using the same antibody clone with 85646-2-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 85646-2-RR (same clone as 85646-2-PBS)

    IF Staining of HeLa using 85646-2-RR (same clone as 85646-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PAK2 antibody (85646-2-RR, Clone: 243124G9 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85646-2-PBS in a different storage buffer formulation.

PAK2 Recombinant monoclonal antibody, PBS Only

PAK2 Uni-rAb® Recombinant Antibody for WB, IF/ICC, IP, Indirect ELISA
Cat No. 85646-2-PBS

产品说明书

CloneNo. 243124G9

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, IP, Indirect ELISA

别名

C t PAK2, EC:2.7.11.1, Gamma PAK, Gamma-PAK, p21 activated kinase 2

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy PAK2 Ready-To-Use IHC Kit. PAK2 primary antibody included.

产品信息

85646-2-PBS targets PAK2 in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 p21 protein (Cdc42/Rac)-activated kinase 2
别名 C t PAK2, EC:2.7.11.1, Gamma PAK, Gamma-PAK, p21 activated kinase 2
计算分子量 58 kDa
观测分子量 54 kDa
GenBank蛋白编号NM_002577
基因名称 PAK2
Gene ID (NCBI) 5062
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ13177
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PAK2, also named as PAK65, PAKgamma, p58, PAK-2p27, PAK-2p24 and C-t-PAK2, belongs to the protein kinase superfamily, STE Ser/Thr protein kinase family and STE20 subfamily. Full length PAK 2 stimulates cell survival and cell growth. The process is, at least in part, mediated by phosphorylation and inhibition of pro-apoptotic BAD. PAK2 has several isoforms with the MW of 54-62 kDa and 41 kDa. The 62 kDa PAK2 is cleaved into a 34 kDa C terminal fragment and a 28 kDa N terminal fragment with a time course that parallels apoptotic death in Jurkat cells. (PMID:10200518). Caspase-activated PAK-2p34 is involved in cell death response, probably involving the JNK signaling pathway. Cleaved PAK-2p34 seems to have a higher activity than the CDC42-activated form.

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