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验证数据展示

  • WB analysis using 51137-1-AP (same clone as 51137-1-PBS)

    WB analysis using 51137-1-AP (same clone as 51137-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 51137-1-AP (PAK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 51137-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 51137-1-AP (same clone as 51137-1-PBS)

    WB analysis of HEK-293 using 51137-1-AP (same clone as 51137-1-PBS)

    WB result of PAK1 antibody (51137-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-PAK1 transfected HEK-293 cells. This data was developed using the same antibody clone with 51137-1-PBS in a different storage buffer formulation.

  • IHC staining of human stomach cancer using 51137-1-AP (same clone as 51137-1-PBS)

    IHC staining of human stomach cancer using 51137-1-AP (same clone as 51137-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer using 51137-1-AP (PAK1 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 51137-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PAK1 antibody included
  • IHC staining of human stomach cancer using 51137-1-AP (same clone as 51137-1-PBS)

    IHC staining of human stomach cancer using 51137-1-AP (same clone as 51137-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer using 51137-1-AP (PAK1 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 51137-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PAK1 antibody included
  • IF Staining of HeLa using 51137-1-AP (same clone as 51137-1-PBS)

    IF Staining of HeLa using 51137-1-AP (same clone as 51137-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PAK1 antibody (51137-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 51137-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

PAK1 Polyclonal antibody, PBS Only

PAK1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 51137-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy PAK1 Ready-To-Use IHC Kit. PAK1 primary antibody included.

产品信息

51137-1-PBS targets PAK1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 p21 protein (Cdc42/Rac)-activated kinase 1
别名
计算分子量 553 aa, 62 kDa
观测分子量 61-65 kDa
GenBank蛋白编号BC109299
基因名称 PAK1
Gene ID (NCBI) 5058
RRIDAB_2283523
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ13153
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The p21-Activated kinase 1 (PAK1), a member of serine-threonine kinases family, was initially identified as an interactor of the Rho GTPases RAC1 and CDC42, which affect a wide range of processes associated with cell motility, survival, metabolism, cell cycle, proliferation, transformation, stress, inflammation, and gene expression(PMID: 32863957). PAK1 is over-expressed in various malignancies such as ovarian, breast, lung, and bladder cancers, and is closely associated with tumor invasion.

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