验证数据展示
经过测试的应用
| Positive WB detected in | A431 cells, HeLa cells, Jurkat cells, mouse lung tissue |
| Positive IP detected in | A431 cells |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
15583-1-AP targets PAIP2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag7119 Product name: Recombinant human PAIP2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-127 aa of BC001716 Sequence: MKDPSRSSTSPSIINEDVIINGHSHEDDNPFAEYMWMENEEEFNRQIEEELWEEEFIERCFQEMLEEEEEHEWFIPARDLPQTMDQIQDQFNDLVISDGSSLEDLVVKSNLNPNAKEFVPGVKYGNI 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | poly(A) binding protein interacting protein 2 |
| 别名 | PAIP2A, PAIP-2, PAIP 2, PABP-interacting protein 2, PABP interacting protein 2 |
| 计算分子量 | 15 kDa |
| 观测分子量 | 26 kDa |
| GenBank蛋白编号 | BC001716 |
| 基因名称 | PAIP2 |
| Gene ID (NCBI) | 51247 |
| RRID | AB_10950891 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9BPZ3 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PAIP2 antibody 15583-1-AP | Download protocol |
| IF protocol for PAIP2 antibody 15583-1-AP | Download protocol |
| IP protocol for PAIP2 antibody 15583-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Neurosci Proteasome modulates positive and negative translational regulators in long-term synaptic plasticity. | ||
Front Genet PAIP2 is a potential diagnostic and prognostic biomarker of breast cancer and is associated with immune infiltration | ||
Pharmacol Res Inhibition of K63 ubiquitination by G-Protein pathway suppressor 2 (GPS2) regulates mitochondria-associated translation |
