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CoraLite®594-conjugated PAI-1 Monoclonal antibody

PAI-1 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

human, pig

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1H4A5

Cat No : CL594-66261

Print datasheet

Synonyms

PAI, PAI 1, PAI1, PLANH1, Serpin E1, SERPINE1

验证数据展示

  • IF Staining of HepG2 using CL594-66261

    IF Staining of HepG2 using CL594-66261

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite®594-conjugated PAI-1 antibody (CL594-66261, Clone: 1H4A5 ) at dilution of 1:100.

经过测试的应用

Positive IF detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy SERPINE1 Ready-To-Use IHC Kit. SERPINE1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66261-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66261 targets PAI-1 in IF applications and shows reactivity with human, pig samples.

Tested Applications IF/ICC
Tested Reactivity human, pig
Immunogen PAI-1 fusion protein Ag22411 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1
Synonyms PAI, PAI 1, PAI1, PLANH1, Serpin E1, SERPINE1
Calculated Molecular Weight 402 aa, 45 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC010860
Gene Symbol SERPINE1
Gene ID (NCBI) 5054
RRIDAB_2883545
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP05121
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SERPINE1, also named as Plasminogen activator inhibitor type 1 (PAI-1), is a member of the serine protease inhibitor (SERPIN) superfamily. PAI-1 is the principal inhibitor of tissue plasminogen activator (tPA) and uPA, and hence is an inhibitor of fibrinolysis. Defects in this gene are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1 deficiency), and high concentrations of the gene product are associated with thrombophilia.

实验方案

Product Specific Protocols
IF protocol for CL594 PAI-1 antibody CL594-66261Download protocol
Standard Protocols
Click here to view our Standard Protocols