验证数据展示
经过测试的应用
Positive WB detected in | HepG2 cells, mouse kidney tissue |
Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 3 publications below |
IHC | See 1 publications below |
产品信息
16347-1-AP targets PAH in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
经测试应用 | WB, IHC, ELISA Application Description |
文献引用应用 | WB, IHC |
经测试反应性 | human, mouse |
文献引用反应性 | human, mouse |
免疫原 | PAH fusion protein Ag9541 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | phenylalanine hydroxylase |
别名 | PAH, PH, Phe 4 monooxygenase, Phenylalanine 4 hydroxylase, phenylalanine hydroxylase, PKU, PKU1 |
计算分子量 | 452 aa, 52 kDa |
观测分子量 | 52 kDa |
GenBank蛋白编号 | BC026251 |
基因名称 | PAH |
Gene ID (NCBI) | 5053 |
RRID | AB_2878247 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | P00439 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PAH, also known as PH, belongs to the aromatic amino acid hydroxylase (AAAH) enzyme family. PAH catalyzes the conversion of L-phenylalanine (L-Phe) to L-tyrosine (L-Tyr) by para-hydroxylation of the aromatic side chain. PAH is primarily present in the liver, where removal of excess L-Phe prevents the neurotoxic effect of hyperphenylalaninemia (HPA). The calculated molecular weight of PAH is 52 kDa (PMID: 23457044).
实验方案
Product Specific Protocols | |
---|---|
WB protocol for PAH antibody 16347-1-AP | Download protocol |
IHC protocol for PAH antibody 16347-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Pharmacol Res Medioresinol as a novel PGC-1α activator prevents pyroptosis of endothelial cells in ischemic stroke through PPARα-GOT1 axis. | ||
Life Sci Alliance A phosphoproteomic approach reveals that PKD3 controls PKA-mediated glucose and tyrosine metabolism. | ||
Adv Sci (Weinh) A Metabolic Reprogramming Amino Acid Polymer as an Immunosurveillance Activator and Leukemia Targeting Drug Carrier for T‐Cell Acute Lymphoblastic Leukemia | ||
Arthritis Res Ther Investigation of ferroptosis-associated molecular subtypes and immunological characteristics in lupus nephritis based on artificial neural network learning |