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验证数据展示

  • WB analysis using 83868-1-RR (same clone as 83868-1-PBS)

    WB analysis using 83868-1-RR (same clone as 83868-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83868-1-RR (PADI2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83868-1-PBS in a different storage buffer formulation.

  • IHC staining of human ovarian cancer using 83868-1-RR (same clone as 83868-1-PBS)

    IHC staining of human ovarian cancer using 83868-1-RR (same clone as 83868-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 83868-1-RR (PADI2 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83868-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83868-1-RR

    Affinity and Kinetic Characterization of 83868-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83868-1-RR against Human PADI2 were performed. The affinity constant is 1.01 nM.

PADI2 Recombinant monoclonal antibody, PBS Only

PADI2 Uni-rAb® Recombinant Antibody for WB, IHC, ELISA
Cat No. 83868-1-PBS

产品说明书

CloneNo. 240763C5

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

240763C5, EC:3.5.3.15, KIAA0994, PAD H19, PAD2

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

83868-1-PBS targets PADI2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag17612

Product name: Recombinant human PADI2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 118-438 aa of BC009701

Sequence: ISLDVDADRDGVVEKNNPKKASWTWGPEGQGAILLVNCDRETPWLPKEDCRDEKVYSKEDLKDMSQMILRTKGPDRLPAGYEIVLYISMSDSDKVGVFYVENPFFGQRYIHILGRRKLYHVVKYTGGSAELLFFVEGLCFPDEGFSGLVSIHVSLLEYMAQDIPLTPIFTDTVIFRIAPWIMTPNILPPVSVFVCCMKDNYLFLKEVKNLVEKTNCELKVCFQYLNRGDRWIQDEIEFGYIEAPHKGFPVVLDSPRDGNLKDFPVKELLGPDFGYVTREPLFESVTSLDSFGNLEVSPPVTVNGKTYPLGRILIGSSFPL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 peptidyl arginine deiminase, type II
别名 240763C5, EC:3.5.3.15, KIAA0994, PAD H19, PAD2
计算分子量 665 aa, 75 kDa
观测分子量75 kDa
GenBank蛋白编号BC009701
基因名称 PADI2
Gene ID (NCBI) 11240
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ9Y2J8
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PADI2, also named KIAA0994, PDI2, PAD-H19, and PAD2(Peptidylarginine deiminase II ), belongs to the protein arginine deiminase family. It catalyzes the deimination of arginine residues of proteins. PADI2 may play a regulatory role in the expression of lactation-related genes via histone citrullination during diestrus (PMID:20668670).

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