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验证数据展示

  • WB analysis using 80294-1-RR (same clone as 80294-1-PBS)

    WB analysis using 80294-1-RR (same clone as 80294-1-PBS)

    Lysates of HepG2 cells treated with Chloroquine or not were subjected to SDS PAGE followed by western blot with 80294-1-RR (P62,SQSTM1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80294-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 80294-1-RR (same clone as 80294-1-PBS)

    WB analysis of HEK-293 using 80294-1-RR (same clone as 80294-1-PBS)

    WB result of P62,SQSTM1 antibody (80294-1-RR; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-P62,SQSTM1 transfected HEK-293 cells. This data was developed using the same antibody clone with 80294-1-PBS in a different storage buffer formulation.

  • WB analysis using 80294-1-RR (same clone as 80294-1-PBS)

    WB analysis using 80294-1-RR (same clone as 80294-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 80294-1-RR (P62,SQSTM1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80294-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 80294-1-RR (same clone as 80294-1-PBS)

    IF Staining of HeLa using 80294-1-RR (same clone as 80294-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed Chloroquine treated HeLa cells using P62,SQSTM1 antibody (80294-1-RR, Clone: 1D17 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 80294-1-PBS in a different storage buffer formulation.

  • FC experiment of Jurkat using 80294-1-RR (same clone as 80294-1-PBS)

    FC experiment of Jurkat using 80294-1-RR (same clone as 80294-1-PBS)

    1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human P62,SQSTM1 (80294-1-RR, Clone:1D17) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 80294-1-PBS in a different storage buffer formulation.

  • IP experiment of HEK-293 using 80294-1-RR (same clone as 80294-1-PBS)

    IP experiment of HEK-293 using 80294-1-RR (same clone as 80294-1-PBS)

    IP result of anti-P62,SQSTM1 (IP:80294-1-RR, 4ug; Detection:80294-1-RR 1:500) with HEK-293 cells lysate 1640 ug. This data was developed using the same antibody clone with 80294-1-PBS in a different storage buffer formulation.

  • IHC staining of human pancreas cancer using 80294-1-RR (same clone as 80294-1-PBS)

    IHC staining of human pancreas cancer using 80294-1-RR (same clone as 80294-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 80294-1-RR (P62,SQSTM1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80294-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with P62,SQSTM1 antibody included
  • Featured Product
  • KD/KO验证

P62,SQSTM1 Recombinant antibody, PBS Only

P62,SQSTM1 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA
Cat No. 80294-1-PBS

产品说明书

CloneNo. 1D17

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

别名

P62/SQSTM1, P62, SQSTM1, Sequestosome-1, Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa

缓冲液配方:  PBS Only
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy P62,SQSTM1 Ready-To-Use IHC Kit. P62,SQSTM1 primary antibody included.

产品信息

80294-1-PBS targets P62,SQSTM1 in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA Application Description
经测试反应性 human
免疫原 P62,SQSTM1 fusion protein Ag13131 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 sequestosome 1
别名 P62/SQSTM1, P62, SQSTM1, Sequestosome-1, Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa
计算分子量 48 kDa
观测分子量62 kDa
GenBank蛋白编号BC017222
基因名称 P62/SQSTM1
Gene ID (NCBI) 8878
RRIDAB_2918883
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ13501
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Sequestosome 1 (SQSTM1/p62) is a multifunctional adaptor protein implicated in selective autophagy, cell signaling pathways, and tumorigenesis. p62 has been implicated in shuttling ubiquitinated and aggregated proteins for autophagic degradation. p62 is degraded during the autophagic process, which makes its intracellular level a marker for autophagy progression. p62 is at the cross-roads of several signaling pathways including Ras/ Raf/ MAPK and NFκB and plays important role in cancer. p62 is a component of inclusion bodies/ protein aggregates found in human diseases, including Huntington's disease, Alzheimer's disease, Parkinson's disease, and nephropathic cystinosis (PMID: 22074114, 22860231, 22714671).