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验证数据展示

  • WB analysis using 80077-1-RR (same clone as 80077-1-PBS)

    WB analysis using 80077-1-RR (same clone as 80077-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 80077-1-RR (P53 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80077-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 80077-1-RR (same clone as 80077-1-PBS)

    IF Staining of HepG2 using 80077-1-RR (same clone as 80077-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using P53 antibody (80077-1-RR, Clone: 2H13 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 80077-1-PBS in a different storage buffer formulation.

  • IHC staining of human ovarian cancer using 80077-1-RR (same clone as 80077-1-PBS)

    IHC staining of human ovarian cancer using 80077-1-RR (same clone as 80077-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue slide using 80077-1-RR (p53 antibody) at dilution of 1:1000 (under 10x lens).Heat mediated antigen retrieval with Tris-EDTA buffer (pH 7.0). Staining was performed on the Epredia 480S Autostainer.​ This data was developed using the same antibody clone with 80077-1-PBS in a different storage buffer formulation.

  • IHC staining of human ovarian cancer using 80077-1-RR (same clone as 80077-1-PBS)

    IHC staining of human ovarian cancer using 80077-1-RR (same clone as 80077-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue slide using 80077-1-RR (p53 antibody) at dilution of 1:1000 (under 40x lens).Heat mediated antigen retrieval with Tris-EDTA buffer (pH 7.0). Staining was performed on the Epredia 480S Autostainer. This data was developed using the same antibody clone with 80077-1-PBS in a different storage buffer formulation.

  • WB analysis of zebrafish using 80077-1-RR (same clone as 80077-1-PBS)

    WB analysis of zebrafish using 80077-1-RR (same clone as 80077-1-PBS)

    zebrafish tissue were subjected to SDS PAGE followed by western blot with 80077-1-RR (P53 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80077-1-PBS in a different storage buffer formulation.

  • WB analysis of A431 using 80077-1-RR (same clone as 80077-1-PBS)

    WB analysis of A431 using 80077-1-RR (same clone as 80077-1-PBS)

    WB result of P53 antibody (80077-1-RR; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-P53 transfected A431 cells. This data was developed using the same antibody clone with 80077-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 80077-1-RR (same clone as 80077-1-PBS)

    IHC staining of human colon cancer using 80077-1-RR (same clone as 80077-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 80077-1-RR (P53 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80077-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with P53 antibody included
  • IF Staining of A431 using 80077-1-RR (same clone as 80077-1-PBS)

    IF Staining of A431 using 80077-1-RR (same clone as 80077-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using P53 antibody (80077-1-RR, Clone: 2H13 ) at dilution of 1:500 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR004). This data was developed using the same antibody clone with 80077-1-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 80077-1-RR (same clone as 80077-1-PBS)

    FC experiment of HepG2 using 80077-1-RR (same clone as 80077-1-PBS)

    1X10^6 HepG2 cells were intracellularly stained with 0.5 ug Anti-Human P53 (80077-1-RR, Clone:2H13) (red) labeled with FlexAble CoraLite®488 Antibody Labeling Kit for Rabbit IgG (KFA001), or 0.5 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 80077-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

P53 Recombinant monoclonal antibody, PBS Only

P53 Uni-rAb® Recombinant Antibody for WB, IHC-Autostainer, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 80077-1-PBS

产品说明书

CloneNo. 2H13

宿主/亚型

Rabbit / IgG

种属反应性

human, zebrafish

应用

WB, IHC-Autostainer, IF/ICC, FC (Intra), Indirect ELISA

别名

TP53, 2H13, Antigen NY-CO-13, tumor protein p53, Tumor suppressor p53

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy P53 Ready-To-Use IHC Kit. P53 primary antibody included.

产品信息

80077-1-PBS targets P53 in WB, IHC-Autostainer, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, zebrafish samples.

经测试应用 WB, IHC-Autostainer, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, zebrafish
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 tumor protein p53
别名 TP53, 2H13, Antigen NY-CO-13, tumor protein p53, Tumor suppressor p53
计算分子量 44 kDa
观测分子量53 kDa
GenBank蛋白编号BC003596
基因名称 P53
Gene ID (NCBI) 7157
RRIDAB_2918862
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP04637
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TP53, also known as P53 and NY-CO-13, belongs to the p53 family and has 9 isoforms. In SDS-Page, the observed molecular weight is about 53 kDa. TP53 acts as a tumor suppressor in many tumor types, including growth arrest or apoptosis depending on the physiological circumstances and cell types. It is involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. TP53 Localizes in the nucleus in most cells but found in the cytoplasm in some cells. (PMID: 26166714; PMID: 25225161)

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