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验证数据展示

  • WB analysis using 66101-1-Ig (same clone as 66101-1-PBS)

    WB analysis using 66101-1-Ig (same clone as 66101-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66101-1-Ig (P4HA1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66101-1-PBS in a different storage buffer formulation.

  • WB analysis of PC-3 using 66101-1-Ig (same clone as 66101-1-PBS)

    WB analysis of PC-3 using 66101-1-Ig (same clone as 66101-1-PBS)

    PC-3 cells were subjected to SDS PAGE followed by western blot with 66101-1-Ig (P4HA1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66101-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 66101-1-Ig (same clone as 66101-1-PBS)

    IHC staining of human breast cancer using 66101-1-Ig (same clone as 66101-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66101-1-Ig (P4HA1 Antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66101-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with P4HA1 antibody included
  • IHC staining of human breast cancer using 66101-1-Ig (same clone as 66101-1-PBS)

    IHC staining of human breast cancer using 66101-1-Ig (same clone as 66101-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66101-1-Ig (P4HA1 Antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66101-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with P4HA1 antibody included
  • Featured Product
  • KD/KO验证

P4HA1 Monoclonal antibody, PBS Only

P4HA1 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 66101-1-PBS

产品说明书

CloneNo. 1H10A6

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

Prolyl 4-hydroxylase subunit alpha-1, Procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-1, P4HA, EC:1.14.11.2, 1H10A6

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy P4HA1 Ready-To-Use IHC Kit. P4HA1 primary antibody included.

产品信息

66101-1-PBS targets P4HA1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 P4HA1 fusion protein Ag17867 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 prolyl 4-hydroxylase, alpha polypeptide I
别名 Prolyl 4-hydroxylase subunit alpha-1, Procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-1, P4HA, EC:1.14.11.2, 1H10A6
计算分子量 534 aa, 61 kDa
观测分子量 61 kDa
GenBank蛋白编号BC034998
基因名称 P4HA1
Gene ID (NCBI) 5033
RRIDAB_2881500
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP13674
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

P4HA1(Prolyl 4-hydroxylase subunit alpha-1) is also named as P4HA and belongs to the P4HA family , which play a central role in collagen synthesis. P4HA1 catalyzes the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins. The gene encodes a polypeptide of 517 amino acid residues and a signal peptide of 17 amino acids and the full length protein has two glycosylation sites (uniprot). It can exsit as a heteromer, dimer or tetramer (refer to: GENATLAS). P4HA1 has 3 isoforms produced by alternative splicing with the molecular weight of 61 kDa, 61 kDa and 59 kDa. This antibody is specific to P4HA1.