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验证数据展示

  • WB analysis using 66101-1-Ig (same clone as 66101-1-PBS)

    WB analysis using 66101-1-Ig (same clone as 66101-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66101-1-Ig (P4HA1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66101-1-PBS in a different storage buffer formulation.

  • WB analysis of PC-3 using 66101-1-Ig (same clone as 66101-1-PBS)

    WB analysis of PC-3 using 66101-1-Ig (same clone as 66101-1-PBS)

    PC-3 cells were subjected to SDS PAGE followed by western blot with 66101-1-Ig (P4HA1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66101-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 66101-1-Ig (same clone as 66101-1-PBS)

    IHC staining of human breast cancer using 66101-1-Ig (same clone as 66101-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66101-1-Ig (P4HA1 Antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66101-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with P4HA1 antibody included
  • IHC staining of human breast cancer using 66101-1-Ig (same clone as 66101-1-PBS)

    IHC staining of human breast cancer using 66101-1-Ig (same clone as 66101-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66101-1-Ig (P4HA1 Antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66101-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with P4HA1 antibody included
  • Featured Product
  • KD/KO验证

P4HA1 Monoclonal antibody, PBS Only

P4HA1 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 66101-1-PBS

产品说明书

CloneNo. 1H10A6

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

1H10A6, EC:1.14.11.2, P4HA, Procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-1, Prolyl 4-hydroxylase subunit alpha-1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy P4HA1 Ready-To-Use IHC Kit. P4HA1 primary antibody included.

产品信息

66101-1-PBS targets P4HA1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag17867

Product name: Recombinant human P4HA1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 236-534 aa of BC034998

Sequence: PEHQRANGNLKYFEYIMAKEKDVNKSASDDQSDQKTTPKKKGVAVDYLPERQKYEMLCRGEGIKMTPRRQKKLFCRYHDGNRNPKFILAPAKQEDEWDKPRIIRFHDIISDAEIEIVKDLAKPRLRRATISNPITGDLETVHYRISKSAWLSGYENPVVSRINMRIQDLTGLDVSTAEELQVANYGVGGQYEPHFDFARKDEPDAFKELGTGNRIATWLFYMSDVSAGGATVFPEVGASVWPKKGTAVFWYNLFASGEGDYSTRHAACPVLVGNKWVSNKWLHERGQEFRRPCTLSELE

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 prolyl 4-hydroxylase, alpha polypeptide I
别名 1H10A6, EC:1.14.11.2, P4HA, Procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-1, Prolyl 4-hydroxylase subunit alpha-1
计算分子量 534 aa, 61 kDa
观测分子量 61 kDa
GenBank蛋白编号BC034998
基因名称 P4HA1
Gene ID (NCBI) 5033
RRIDAB_2881500
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP13674
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

P4HA1(Prolyl 4-hydroxylase subunit alpha-1) is also named as P4HA and belongs to the P4HA family , which play a central role in collagen synthesis. P4HA1 catalyzes the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins. The gene encodes a polypeptide of 517 amino acid residues and a signal peptide of 17 amino acids and the full length protein has two glycosylation sites (uniprot). It can exsit as a heteromer, dimer or tetramer (refer to: GENATLAS). P4HA1 has 3 isoforms produced by alternative splicing with the molecular weight of 61 kDa, 61 kDa and 59 kDa. This antibody is specific to P4HA1.