验证数据展示
经过测试的应用
Positive WB detected in | PC-3 cells, HEK-293 cells, HeLa cells, HSC-T6 cells, ROS1728 cells, Neuro-2a cells, 4T1 cells, rat liver tissue, NIH/3T3 cells, MCF-7 cells |
Positive IHC detected in | human breast cancer tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:200-1:1000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
KD/KO | See 2 publications below |
WB | See 3 publications below |
IHC | See 1 publications below |
产品信息
66101-1-Ig targets P4HA1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, IHC, ELISA Application Description |
文献引用应用 | WB, IHC |
经测试反应性 | human, mouse, rat |
文献引用反应性 | human |
免疫原 | P4HA1 fusion protein Ag17867 种属同源性预测 |
宿主/亚型 | Mouse / IgG1 |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | prolyl 4-hydroxylase, alpha polypeptide I |
别名 | Prolyl 4-hydroxylase subunit alpha-1, Procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-1, P4HA, EC:1.14.11.2, 1H10A6 |
计算分子量 | 534 aa, 61 kDa |
观测分子量 | 61 kDa |
GenBank蛋白编号 | BC034998 |
基因名称 | P4HA1 |
Gene ID (NCBI) | 5033 |
RRID | AB_2881500 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purification |
UNIPROT ID | P13674 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
P4HA1(Prolyl 4-hydroxylase subunit alpha-1) is also named as P4HA and belongs to the P4HA family , which play a central role in collagen synthesis. P4HA1 catalyzes the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins. The gene encodes a polypeptide of 517 amino acid residues and a signal peptide of 17 amino acids and the full length protein has two glycosylation sites (uniprot). It can exsit as a heteromer, dimer or tetramer (refer to: GENATLAS). P4HA1 has 3 isoforms produced by alternative splicing with the molecular weight of 61 kDa, 61 kDa and 59 kDa. This antibody is specific to P4HA1.
实验方案
Product Specific Protocols | |
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WB protocol for P4HA1 antibody 66101-1-Ig | Download protocol |
IHC protocol for P4HA1 antibody 66101-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
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Mol Oncol Prolyl 4-hydroxylase subunit alpha 1 (P4HA1) is a biomarker of poor prognosis in primary melanomas, and its depletion inhibits melanoma cell invasion and disrupts tumor blood vessel walls.
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Front Oncol P4HA1 Regulates CD31 via COL6A1 in the Transition of Glioblastoma Stem-Like Cells to Tumor Endothelioid Cells.
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Front Mol Biosci Identification of Prognostic Biomarkers in Patients With Malignant Rhabdoid Tumor of the Kidney Based on mTORC1 Signaling Pathway-Related Genes. |