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P4HA1 Monoclonal antibody

P4HA1 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 66101-1-Ig

产品说明书

CloneNo. 1H10A6

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

Prolyl 4-hydroxylase subunit alpha-1, Procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-1, P4HA, EC:1.14.11.2, 1H10A6

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite®594
规格: 

-/ -


经过测试的应用

Positive WB detected inPC-3 cells, HEK-293 cells, HeLa cells, HSC-T6 cells, ROS1728 cells, Neuro-2a cells, 4T1 cells, rat liver tissue, NIH/3T3 cells, MCF-7 cells
Positive IHC detected inhuman breast cancer tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy P4HA1 Ready-To-Use IHC Kit. P4HA1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66101-1-Ig targets P4HA1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 P4HA1 fusion protein Ag17867 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 prolyl 4-hydroxylase, alpha polypeptide I
别名 Prolyl 4-hydroxylase subunit alpha-1, Procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-1, P4HA, EC:1.14.11.2, 1H10A6
计算分子量 534 aa, 61 kDa
观测分子量 61 kDa
GenBank蛋白编号BC034998
基因名称 P4HA1
Gene ID (NCBI) 5033
RRIDAB_2881500
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP13674
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

P4HA1(Prolyl 4-hydroxylase subunit alpha-1) is also named as P4HA and belongs to the P4HA family , which play a central role in collagen synthesis. P4HA1 catalyzes the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins. The gene encodes a polypeptide of 517 amino acid residues and a signal peptide of 17 amino acids and the full length protein has two glycosylation sites (uniprot). It can exsit as a heteromer, dimer or tetramer (refer to: GENATLAS). P4HA1 has 3 isoforms produced by alternative splicing with the molecular weight of 61 kDa, 61 kDa and 59 kDa. This antibody is specific to P4HA1.

实验方案

Product Specific Protocols
WB protocol for P4HA1 antibody 66101-1-IgDownload protocol
IHC protocol for P4HA1 antibody 66101-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC

Mol Oncol

Prolyl 4-hydroxylase subunit alpha 1 (P4HA1) is a biomarker of poor prognosis in primary melanomas, and its depletion inhibits melanoma cell invasion and disrupts tumor blood vessel walls.

Authors - Johanna Eriksson
  • KD Validated
humanWB

Front Oncol

P4HA1 Regulates CD31 via COL6A1 in the Transition of Glioblastoma Stem-Like Cells to Tumor Endothelioid Cells.

Authors - Xiangming Han
  • KD Validated
humanWB

Front Mol Biosci

Identification of Prognostic Biomarkers in Patients With Malignant Rhabdoid Tumor of the Kidney Based on mTORC1 Signaling Pathway-Related Genes.

Authors - Chenghao Zhanghuang
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