验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells | 
推荐稀释比
| 应用 | 推荐稀释比 | 
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 | 
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below | 
产品信息
11300-1-AP targets P2RY6 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description | 
| 文献引用应用 | WB | 
| 经测试反应性 | human, mouse, rat | 
| 文献引用反应性 | human | 
| 免疫原 | CatNo: Ag1820 Product name: Recombinant human P2RY6 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-328 aa of BC009391 Sequence: MEWDNGTGQALGLPPTTCVYRENFKQLLLPPVYSAVLAAGLPLNICVITQICTSRRALTRTAVYTLNLALADLLYACSLPLLIYNYAQGDHWPFGDFACRLVRFLFYANLHGSILFLTCISFQRYLGICHPLAPWHKRGGRRAAWLVCVAVWLAVTTQCLPTAIFAATGIQRNRTVCYDLSPPALATHYMPYGMALTVIGFLLPFAALLACYCLLACRLCRQDGPAEPVAQERRGKAARMAVVVAAAFAISFLPFHITKTAYLAVRSTPGVPCTVLEAFAAAYKGTRPFASANSVLDPILFYFTQKKFRRRPHELLQKLTAKWQRQGR种属同源性预测 | 
| 宿主/亚型 | Rabbit / IgG | 
| 抗体类别 | Polyclonal | 
| 产品类型 | Antibody | 
| 全称 | pyrimidinergic receptor P2Y, G-protein coupled, 6 | 
| 别名 | P2RY6, P2Y purinoceptor 6, P2Y6 | 
| 计算分子量 | 36 kDa | 
| 观测分子量 | 45 kDa | 
| GenBank蛋白编号 | BC009391 | 
| 基因名称 | P2RY6 | 
| Gene ID (NCBI) | 5031 | 
| RRID | AB_2283156 | 
| 偶联类型 | Unconjugated | 
| 形式 | Liquid | 
| 纯化方式 | Antigen affinity purification | 
| UNIPROT ID | Q15077 | 
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. | 
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. | 
背景介绍
Nucleotides, the structural subunits of the nucleic acids, are also important extracellular signaling molecules. P2 receptors are a family of cell surface receptors that mediate a wide variety of physiologic effects in response to extracellular nucleotides. These receptors fall into two classes: P2X receptors, which are ligand-gated ion channels that mediate calcium and potassium fluxes in response to ATP, and P2Y receptors, which are G protein-coupled receptors (GPCRs). P2RY6 is responsive to UDP, partially responsive to UTP and ADP, and not responsive to ATP. The activity of this receptor is mediated by G proteins which activate a phosphatidylinositol-calcium second messenger system. (PMID: 8670200; 21724990)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for P2RY6 antibody 11300-1-AP | Download protocol | 
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols | 
发表文章
| Species | Application | Title | 
|---|---|---|
| Radiat Res Involvement of P2Y6 Receptor in p38 MAPK-Mediated COX-2 Expression in Response to UVB Irradiation of Human Keratinocytes. | ||
| Radiat Res Involvement of P2Y6 receptor in p38 MAPK-mediated COX-2 expression in response to UVB irradiation of human keratinocytes. | 

