• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 20335-1-AP

    WB analysis using 20335-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20335-1-AP (P2RY13 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human small intestine using 20335-1-AP

    IHC staining of human small intestine using 20335-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 20335-1-AP (P2RY13 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human small intestine using 20335-1-AP

    IHC staining of human small intestine using 20335-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 20335-1-AP (P2RY13 Antibody) at dilution of 1:50 (under 40x lens).

P2RY13 Polyclonal antibody

P2RY13 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 20335-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

FKSG77, GPCR1, GPR86, GPR94, G-protein coupled receptor 86

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inHepG2 cells
Positive IHC detected inhuman small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20335-1-AP targets P2RY13 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human
文献引用反应性human
免疫原 P2RY13 fusion protein Ag14108 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 purinergic receptor P2Y, G-protein coupled, 13
别名 FKSG77, GPCR1, GPR86, GPR94, G-protein coupled receptor 86
计算分子量 354 aa, 41 kDa
观测分子量 45 kDa
GenBank蛋白编号BC041116
基因名称 P2RY13
Gene ID (NCBI) 53829
RRIDAB_2878674
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BPV8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

P2RY13 (also named GPR86 or GPR94) is a G protein-coupled receptor (GPCR), which is involved in the pathogenesis of inflammation and immune disorders (PMID: 35982893). Several studies have reported that P2RY13 is a key regulator of cholesterol transport and hepatic HDL endocytosis and is involved in bone formation, remodeling, cell survival, and neuroprotection (PMID: 38045624).

实验方案

Product Specific Protocols
WB protocol for P2RY13 antibody 20335-1-APDownload protocol
IHC protocol for P2RY13 antibody 20335-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Health Sci Rep

P2RY13 is a prognostic biomarker and associated with immune infiltrates in renal clear cell carcinoma: A comprehensive bioinformatic study

Authors - Jie Chu
View Article
humanWB

Nat Methods

SEVtras delineates small extracellular vesicles at droplet resolution from single-cell transcriptomes

Authors - Ruiqiao He
View Article