• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 20335-1-AP

    WB analysis using 20335-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20335-1-AP (P2RY13 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human small intestine using 20335-1-AP

    IHC staining of human small intestine using 20335-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 20335-1-AP (P2RY13 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human small intestine using 20335-1-AP

    IHC staining of human small intestine using 20335-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 20335-1-AP (P2RY13 Antibody) at dilution of 1:50 (under 40x lens).

P2RY13 Polyclonal antibody

P2RY13 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 20335-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

FKSG77, GPCR1, GPR86, GPR94, G-protein coupled receptor 86

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inHepG2 cells
Positive IHC detected inhuman small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20335-1-AP targets P2RY13 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human
文献引用反应性human
免疫原

CatNo: Ag14108

Product name: Recombinant human P2RY13 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 253-333 aa of BC041116

Sequence: ARVPYTHSQTNNKTDCRLQNQLFIAKETTLFLAATNICMDPLIYIFLCKKFTEKLPCMQGRKTTASSQENHSSQTDNITLG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 purinergic receptor P2Y, G-protein coupled, 13
别名 FKSG77, GPCR1, GPR86, GPR94, G-protein coupled receptor 86
计算分子量 354 aa, 41 kDa
观测分子量 45 kDa
GenBank蛋白编号BC041116
基因名称 P2RY13
Gene ID (NCBI) 53829
RRIDAB_2878674
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BPV8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

P2RY13 (also named GPR86 or GPR94) is a G protein-coupled receptor (GPCR), which is involved in the pathogenesis of inflammation and immune disorders (PMID: 35982893). Several studies have reported that P2RY13 is a key regulator of cholesterol transport and hepatic HDL endocytosis and is involved in bone formation, remodeling, cell survival, and neuroprotection (PMID: 38045624).

实验方案

Product Specific Protocols
WB protocol for P2RY13 antibody 20335-1-APDownload protocol
IHC protocol for P2RY13 antibody 20335-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Health Sci Rep

P2RY13 is a prognostic biomarker and associated with immune infiltrates in renal clear cell carcinoma: A comprehensive bioinformatic study

Authors - Jie Chu
View Article
humanWB

Nat Methods

SEVtras delineates small extracellular vesicles at droplet resolution from single-cell transcriptomes

Authors - Ruiqiao He
View Article