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P2RY13 Polyclonal antibody

P2RY13 Polyclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 20335-1-AP

Print datasheet

Synonyms

FKSG77, GPCR1, GPR86, GPR94, P2RY13, P2Y13, SP174

验证数据展示

  • WB analysis of HeLa using 20335-1-AP

    WB analysis of HeLa using 20335-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 20335-1-AP (P2RY13 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human small intestine using 20335-1-AP

    IHC staining of human small intestine using 20335-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 20335-1-AP (P2RY13 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human small intestine using 20335-1-AP

    IHC staining of human small intestine using 20335-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 20335-1-AP (P2RY13 Antibody) at dilution of 1:50 (under 40x lens).

  • FC experiment of HeLa using 20335-1-AP

    FC experiment of HeLa using 20335-1-AP

    1X10^6 HeLa cells were stained with .2ug P2RY13 antibody (20335-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells
Positive IHC detected inhuman small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 20335-1-AP is P2RY13 Fusion Protein expressed in E. coli.

Tested Applications FC, IHC, WB, ELISA
Cited ApplicationsWB, IHC
Tested Reactivity human
Cited Reactivityhuman
Immunogen P2RY13 fusion protein Ag14108 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name purinergic receptor P2Y, G-protein coupled, 13
Synonyms FKSG77, GPCR1, GPR86, GPR94, P2RY13, P2Y13, SP174
Calculated Molecular Weight 354 aa, 41 kDa
Observed Molecular Weight 45 kDa
GenBank Accession NumberBC041116
Gene Symbol P2RY13
Gene ID (NCBI) 53829
RRIDAB_2878674
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BPV8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for P2RY13 antibody 20335-1-APDownload protocol
IHC protocol for P2RY13 antibody 20335-1-APDownload protocol
FC protocol for P2RY13 antibody 20335-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Health Sci Rep

P2RY13 is a prognostic biomarker and associated with immune infiltrates in renal clear cell carcinoma: A comprehensive bioinformatic study

Authors - Jie Chu
View Article
humanWB

Nat Methods

SEVtras delineates small extracellular vesicles at droplet resolution from single-cell transcriptomes

Authors - Ruiqiao He
View Article