P2RX4 Monoclonal antibody
P2RX4 Monoclonal Antibody for FC, IHC, WB, ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
human, mouse, pig, rat
Applications
FC, IHC, WB, ELISA
Conjugate
Unconjugated
CloneNo.
1A5A6
验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, A549 cells, mouse brain tissue, rat brain tissue, rabbit brain tissue, Neuro-2a cells, human brain tissue, pig brain tissue |
| Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC detected in | HeLa cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
66416-1-Ig targets P2RX4 in WB, FC, IHC, ELISA applications and shows reactivity with human, mouse, pig, rat samples.
| Tested Applications | FC, IHC, WB, ELISA |
| Cited Applications | WB |
| Tested Reactivity | human, mouse, pig, rat |
| Cited Reactivity | mouse |
| Immunogen | P2RX4 fusion protein Ag4445 种属同源性预测 |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | purinergic receptor P2X, ligand-gated ion channel, 4 |
| Synonyms | ATP receptor, P2RX4, P2X purinoceptor 4, P2X4, P2X4R, Purinergic receptor |
| Calculated Molecular Weight | 388 aa, 43 kDa |
| Observed Molecular Weight | 50 kDa |
| GenBank Accession Number | BC033826 |
| Gene Symbol | P2RX4 |
| Gene ID (NCBI) | 5025 |
| RRID | AB_2881788 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q99571 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Nucleotides, the structural subunits of the nucleic acids, are also important extracellular signaling molecules. P2 receptors are a family of cell surface receptors that mediate a wide variety of physiologic effects in response to extracellular nucleotides. These receptors fall into two classes: P2X receptors, which are ligand-gated ion channels that mediate calcium and potassium fluxes in response to ATP, and P2Y receptors, which are G protein-coupled receptors (GPCRs). P2RX4 (P2X purinoceptor 4) is a receptor for ATP acting as a ligand gated ion channel. The calculated molecular weight of P2RX4 is 43 kDa, larger apparent molecular weights of 50-80 kDa have been reported, possibly due to post-translational glycosylation (PMID: 24040145, 15262999; 29382907; 12566439).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for P2RX4 antibody 66416-1-Ig | Download protocol |
| IHC protocol for P2RX4 antibody 66416-1-Ig | Download protocol |
| FC protocol for P2RX4 antibody 66416-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Clin Transl Med P2X4 receptor modulates gut inflammation and favours microbial homeostasis in colitis |
