验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy OGN Ready-To-Use IHC Kit. OGN primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 12755-1-AP
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-12755 targets Osteoglycin in IF/ICC applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 | Osteoglycin fusion protein Ag3484 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | osteoglycin |
| 别名 | Mimecan, OG, OGN, OIF, Osteoglycin, Osteoinductive factor, SLRR3A |
| 计算分子量 | 298 aa, 34 kDa |
| 观测分子量 | 34-40 kDa |
| GenBank蛋白编号 | BC037273 |
| 基因名称 | OGN |
| Gene ID (NCBI) | 4969 |
| RRID | AB_3672547 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P20774 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
This gene encodes a member of the small leucine-rich proteoglycan (SLRP) family of proteins. The encoded protein induces ectopic bone formation in conjunction with transforming growth factor beta and may regulate osteoblast differentiation. High expression of the encoded protein may be associated with elevated heart left ventricular mass. Alternative splicing results in multiple transcript variants which forms proteins with different molecular weight.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 Osteoglycin antibody CL488-12755 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |