• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
Use Able AI chat for product recommendations

验证数据展示

  • IF Staining of NIH/3T3 using CL594-23418

    IF Staining of NIH/3T3 using CL594-23418

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using CoraLite®594 Osteocalcin antibody (CL594-23418) at dilution of 1:400.

  • FC experiment of NIH/3T3 using CL594-23418

    FC experiment of NIH/3T3 using CL594-23418

    1X10^6 NIH/3T3 cells were intracellularly stained with 0.5 ug CoraLite®594 Anti-Human Osteocalcin (CL594-23418) (red), or 0.5 ug CoraLite®594-conjugated Rabbit IgG control Rabbit PolyAb (CL594-30000, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

CoraLite®594-conjugated Osteocalcin/OCN Polyclonal antibody

Osteocalcin/OCN Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL594-23418

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IF/ICC, FC (Intra)

别名

BGLAP, Osteocalcin, BGP, Bone Gla protein, Gamma-carboxyglutamic acid-containing protein

缓冲液配方:  PBS, Proclin 300, BSA, Glycerol
PBS, Proclin 300, BSA, Glycerol
偶联物:  CoraLite®594
规格价格库存

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive IF/ICC detected inNIH/3T3 cells
Positive FC (Intra) detected inNIH/3T3 cells
For other applications, we recommend the unconjugated version of this antibody, 23418-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-23418 targets Osteocalcin/OCN in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag20065

Product name: Recombinant human Osteocalcin protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 24-100 aa of BC113432

Sequence: KPSGAESSKGAAFVSKQEGSEVVKRPRRYLYQWLGAPVPYPDPLEPRREVCELNPDCDELADHIGFQEAYRRFYGPV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 bone gamma-carboxyglutamate (gla) protein
别名 BGLAP, Osteocalcin, BGP, Bone Gla protein, Gamma-carboxyglutamic acid-containing protein
计算分子量 100 aa, 11 kDa
GenBank蛋白编号BC113432
基因名称 Osteocalcin
Gene ID (NCBI) 632
RRIDAB_2919870
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
激发激光Yellow-Green Laser (561 nm)
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP02818
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Osteocalcin is a small, highly conserved molecule associated with mineralization of bone matrix. Osteocalcin is specifically expressed in osteoblasts and is the most abundant non-collagenous protein in bone. It regulates the dynamics of new bone formation and bone resorption by interaction with vitamin D, and by influencing the differentiation of osteoblasts. Osteocalcin is also involved in the posttranslational targeting of vitamin K-dependent gamma-carboxylation, which controls blood coagulation.

实验方案

Product Specific Protocols
FC protocol for CL594 Osteocalcin/OCN antibody CL594-23418Download protocol
IF protocol for CL594 Osteocalcin/OCN antibody CL594-23418Download protocol
Standard Protocols
Click here to view our Standard Protocols
||
New chat

Able™

正在加载，请稍候...