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验证数据展示

  • IF Staining of NIH/3T3 using CL594-23418

    IF Staining of NIH/3T3 using CL594-23418

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using CoraLite®594 Osteocalcin antibody (CL594-23418) at dilution of 1:400.

  • FC experiment of NIH/3T3 using CL594-23418

    FC experiment of NIH/3T3 using CL594-23418

    1X10^6 NIH/3T3 cells were intracellularly stained with 0.5 ug CoraLite®594 Anti-Human Osteocalcin (CL594-23418) (red), or 0.5 ug CoraLite®594-conjugated Rabbit IgG control Rabbit PolyAb (CL594-30000, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

CoraLite®594-conjugated Osteocalcin/OCN Polyclonal antibody

Osteocalcin/OCN Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL594-23418

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IF/ICC, FC (Intra)

别名

BGLAP, Osteocalcin, BGP, Bone Gla protein, Gamma-carboxyglutamic acid-containing protein

缓冲液配方:  PBS, Proclin 300, BSA, Glycerol
PBS, Proclin 300, BSA, Glycerol
偶联物:  CoraLite®594
规格价格库存

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经过测试的应用

Positive IF/ICC detected inNIH/3T3 cells
Positive FC (Intra) detected inNIH/3T3 cells
For other applications, we recommend the unconjugated version of this antibody, 23418-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-23418 targets Osteocalcin/OCN in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag20065

Product name: Recombinant human Osteocalcin protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 24-100 aa of BC113432

Sequence: KPSGAESSKGAAFVSKQEGSEVVKRPRRYLYQWLGAPVPYPDPLEPRREVCELNPDCDELADHIGFQEAYRRFYGPV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 bone gamma-carboxyglutamate (gla) protein
别名 BGLAP, Osteocalcin, BGP, Bone Gla protein, Gamma-carboxyglutamic acid-containing protein
计算分子量 100 aa, 11 kDa
GenBank蛋白编号BC113432
基因名称 Osteocalcin
Gene ID (NCBI) 632
RRIDAB_2919870
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP02818
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for CL594 Osteocalcin/OCN antibody CL594-23418Download protocol
Standard Protocols
Click here to view our Standard Protocols
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