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验证数据展示

  • FC experiment of BALB/c mouse splenocytes using PE-98381

    FC experiment of BALB/c mouse splenocytes using PE-98381

    1x10^6 untreated or Anti-IgM and CD40 treated BALB/c mouse splenocytes were surface stained with CoraLite® Plus 647 Anti-Mouse CD19 and 0.25 ug PE Anti-Mouse OX40L/TNFSF4 Rabbit RecAb (PE-98381, Clone:242227B1). Cells were not fixed.

  • FC experiment of BALB/c mouse splenocytes using PE-98381

    FC experiment of BALB/c mouse splenocytes using PE-98381

    1x10^6 Anti-IgM and CD40 treated BALB/c mouse splenocytes were surface stained with 0.25 ug PE Anti-Mouse OX40L/TNFSF4 Rabbit RecAb (PE-98381, Clone: 242227B1) (red), or PE Rabbit IgG Isotype Control RecAb (PE-98136, Clone: 240953C9) (blue). 1x10^6 untreated BALB/c mouse splenocytes were surface stained with 0.25 ug PE Anti-Mouse OX40L/TNFSF4 Rabbit RecAb (PE-98381, Clone: 242227B1)(black, dashed). Cells were not fixed.

PE Anti-Mouse OX40L/TNFSF4 Rabbit Recombinant Antibody

OX40L/TNFSF4 Uni-rAbTM Recombinant Antibody for FC
Cat No. PE-98381

产品说明书

CloneNo. 242227B1

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

FC

别名

CD252, OX40 ligand, OX40L, Tnfsf4, Tumor necrosis factor ligand superfamily member 4

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  PE
规格价格库存

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经过测试的应用

Positive FC detected inAnti-IgM and CD40 treated BALB/c mouse splenocytes

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in a 100 µl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

PE-98381 targets OX40L/TNFSF4 in FC applications and shows reactivity with mouse samples.

经测试应用 FC Application Description
经测试反应性 mouse
免疫原 OX40L/TNFSF4 fusion protein Eg1573 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 tumor necrosis factor (ligand) superfamily, member 4
别名 CD252, OX40 ligand, OX40L, Tnfsf4, Tumor necrosis factor ligand superfamily member 4
计算分子量22kDa
GenBank蛋白编号NM_009452.2
基因名称 Tnfsf4
Gene ID (NCBI) 22164
偶联类型 PE Fluorescent Dye
最大激发/发射波长496 nm, 565 nm / 578 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP43488
储存缓冲液 PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
储存条件Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

The tumour necrosis factor ligand superfamily member 4 gene (TNFSF4, OX40L), which encodes for the T cell costimulatory molecule OX40 ligand, has been identified as a susceptibility gene for the development of systemic lupus erythematosus (SLE). OX40L/TNFSF4/CD252 is a co-stimulatory checkpoint protein expressed by several types of immune and non-immune cells (PMID: 15750594, 19778912). TNFSF4 mRNA is expressed in melanoma cell lines and melanoma samples, including those with low lymphocytic infiltrates, and is not associated with the ulceration status of the primary tumor (PMID: 31501955).

实验方案

Product Specific Protocols
FC protocol for PE OX40L/TNFSF4 antibody PE-98381Download protocol
Standard Protocols
Click here to view our Standard Protocols