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验证数据展示

  • WB analysis of HUVEC using 82794-7-RR (same clone as 82794-7-PBS)

    WB analysis of HUVEC using 82794-7-RR (same clone as 82794-7-PBS)

    HUVEC cells were subjected to SDS PAGE followed by western blot with 82794-7-RR (OX40L/TNFSF4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82794-7-PBS in a different storage buffer formulation.

  • IF Staining of MJ using 82794-7-RR (same clone as 82794-7-PBS)

    IF Staining of MJ using 82794-7-RR (same clone as 82794-7-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MJ cells using OX40L/TNFSF4 antibody (82794-7-RR, Clone: 241813A6 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 82794-7-PBS in a different storage buffer formulation.

  • FC experiment of MJ using 82794-7-RR (same clone as 82794-7-PBS)

    FC experiment of MJ using 82794-7-RR (same clone as 82794-7-PBS)

    1x10^6 MJ cells were surface stained with 0.25 ug OX40L/TNFSF4 Recombinant antibody (82794-7-RR, Clone:241813A6) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were not fixed. This data was developed using the same antibody clone with 82794-7-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 82794-7-RR (same clone as 82794-7-PBS)

    IHC staining of human tonsillitis using 82794-7-RR (same clone as 82794-7-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 82794-7-RR (OX40L/TNFSF4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82794-7-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82794-7-RR

    Affinity and Kinetic Characterization of 82794-7-RR

    Biolayer interferometry (BLl) kinetic assays of 82794-7-RR against Human OX40L/TNFSF4 were performed. The affinity constant is 13.8 pM.

OX40L/TNFSF4 Recombinant monoclonal antibody, PBS Only

OX40L/TNFSF4 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, FC, Indirect ELISA
Cat No. 82794-7-PBS

产品说明书

CloneNo. 241813A6

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, FC, Indirect ELISA

别名

OX40L, TNFSF4, TNFSF4/CD252, 241813A6, OX 40L

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

82794-7-PBS targets OX40L/TNFSF4 in WB, IHC, IF/ICC, FC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, FC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Eg0582

Product name: Recombinant Human OX40L/TNFSF4 protein (His Tag)

Source: mammalian cells-derived, pCDNA3.4-IGSF8(C6)

Tag: N-6*His

Domain: 51-183 aa of NM_003326.5

Sequence: QVSHRYPRIQSIKVQFTEYKKEKGFILTSQKEDEIMKVQNNSVIINCDGFYLISLKGYFSQEVNISLHYQKDEEPLFQLKKVRSVNSLMVASLTYKDKVYLNVTTDNTSLDDFHVNGGELILIHQNPGEFCVL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 tumor necrosis factor (ligand) superfamily, member 4
别名 OX40L, TNFSF4, TNFSF4/CD252, 241813A6, OX 40L
观测分子量30 kDa
GenBank蛋白编号NM_003326.5
基因名称 OX40L/TNFSF4
Gene ID (NCBI) 7292
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDP23510
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C
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