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验证数据展示

  • WB analysis of HeLa using 29622-1-AP (same clone as 29622-1-PBS)

    WB analysis of HeLa using 29622-1-AP (same clone as 29622-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29622-1-AP (OTUD3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29622-1-PBS in a different storage buffer formulation.

  • WB analysis of HCT 116 using 29622-1-AP (same clone as 29622-1-PBS)

    WB analysis of HCT 116 using 29622-1-AP (same clone as 29622-1-PBS)

    WB result of OTUD3 antibody (29622-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-OTUD3 transfected HCT 116 cells. This data was developed using the same antibody clone with 29622-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

OTUD3 Polyclonal antibody, PBS Only

OTUD3 Polyclonal Antibody for WB, Indirect ELISA
Cat No. 29622-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, Indirect ELISA

别名

DUBA4, KIAA0459, OTU domain containing 3, RP11 460G22.1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

29622-1-PBS targets OTUD3 in WB, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag30425

Product name: Recombinant human OTUD3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 192-398 aa of NM_015207

Sequence: NSEAPAHLQTDFQMLHQDESNKREKIKTKGMDSEDDLRDEVEDAVQKVCNATGCSDFNLIVQNLEAENYNIESAIIAVLRMNQGKRNNAEENLEPSGRVLKQCGPLWEEGGSGARIFGNQGLNEGRTENNKAQASPSEENKANKNQLAKVTNKQRREQQWMEKKKRQEERHRHKALESRGSHRDNNRSEAEANTQVTLVKTFAALNI

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 OTU domain containing 3
别名 DUBA4, KIAA0459, OTU domain containing 3, RP11 460G22.1
计算分子量 45 kDa
观测分子量43 kDa
GenBank蛋白编号NM_015207
基因名称 OTUD3
Gene ID (NCBI) 23252
RRIDAB_2918332
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ5T2D3
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

OTUD3 is a kind of deubiquitinating enzyme that hydrolyzes 'Lys-6'- and 'Lys-11'-linked polyubiquitin. It's an important regulator of energy metabolism. Glucose and fatty acids trigger its nuclear translocation by CBP-dependent acetylation. In the nucleus, deubiquitinates and stabilizes the nuclear receptor PPARD regulating the expression of various genes involved in glucose and lipid metabolism and oxidative phosphorylation. The calculated MW of OTUD3 is 45 kDa, 29622-1-AP can detect the band around 43 kDa. (PubMed:23827681, 35675826).

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