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验证数据展示

  • IHC staining of human cervical cancer using 29921-1-AP (same clone as 29921-1-PBS)

    IHC staining of human cervical cancer using 29921-1-AP (same clone as 29921-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 29921-1-AP (OTUD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29921-1-PBS in a different storage buffer formulation.

  • WB analysis using 29921-1-AP (same clone as 29921-1-PBS)

    WB analysis using 29921-1-AP (same clone as 29921-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 29921-1-AP (OTUD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29921-1-PBS in a different storage buffer formulation.

  • WB analysis using 29921-1-AP (same clone as 29921-1-PBS)

    WB analysis using 29921-1-AP (same clone as 29921-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29921-1-AP (OTUD1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29921-1-PBS in a different storage buffer formulation.

  • IHC staining of human ovary tumor using 29921-1-AP (same clone as 29921-1-PBS)

    IHC staining of human ovary tumor using 29921-1-AP (same clone as 29921-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 29921-1-AP (OTUD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29921-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse liver using 29921-1-AP (same clone as 29921-1-PBS)

    IHC staining of mouse liver using 29921-1-AP (same clone as 29921-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 29921-1-AP (OTUD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29921-1-PBS in a different storage buffer formulation.

  • IHC staining of human cervical cancer using 29921-1-AP (same clone as 29921-1-PBS)

    IHC staining of human cervical cancer using 29921-1-AP (same clone as 29921-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 29921-1-AP (OTUD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29921-1-PBS in a different storage buffer formulation.

OTUD1 Polyclonal antibody, PBS Only

OTUD1 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 29921-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

29921-1-PBS targets OTUD1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag31996

Product name: Recombinant human OTUD1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 272-431 aa of

Sequence: SSAAEPVIVSRSDPRDEKLALYLAEVEKQDKYLRQRNKYRFHIIPDGNCLYRAVSKTVYGDQSLHRELREQTVHYIADHLDHFSPLIEGDVGEFIIAAAQDGAWAGYPELLAMGQMLNVNIHLTTGGRLESPTVSTMIHYLGPEDSLRPSIWLSWLSNGH

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 OTU domain containing 1
别名
计算分子量51 kDa
观测分子量50-55 kDa
基因名称 OTUD1
Gene ID (NCBI) 220213
RRIDAB_3086190
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ5VV17
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

OTUD1, OTU domain-containing protein 1, is a deubiquitinating enzyme that specifically hydrolyzes 'Lys-63'-linked polyubiquitin to monoubiquitin (PMID: 23827681). OTUD1-mediated deubiquitination prevents activation of the ribosome quality control and subsequent dissociation of ribosomes and stimulates formation of polysomes and translation (PMID: 36445135). OTUD1 is a colon-specific deubiquitinase that expressed in normal colonic epithelium goblet cells (PMID: 36910614).

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