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验证数据展示

  • IHC staining of human cervical cancer using 29921-1-AP

    IHC staining of human cervical cancer using 29921-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 29921-1-AP (OTUD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis using 29921-1-AP

    WB analysis using 29921-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 29921-1-AP (OTUD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis using 29921-1-AP

    WB analysis using 29921-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29921-1-AP (OTUD1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary tumor using 29921-1-AP

    IHC staining of human ovary tumor using 29921-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 29921-1-AP (OTUD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse liver using 29921-1-AP

    IHC staining of mouse liver using 29921-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 29921-1-AP (OTUD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human cervical cancer using 29921-1-AP

    IHC staining of human cervical cancer using 29921-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 29921-1-AP (OTUD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

OTUD1 Polyclonal antibody

OTUD1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 29921-1-AP

产品说明书

引用文献(4)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse and More (1)

应用

WB, IHC, ELISA and More (1)

别名

OTU domain containing 1, OTDC1, DUBA7, DUBA 7

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse colon tissue, A549 cells
Positive IHC detected inhuman cervical cancer tissue, human ovary tumor tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29921-1-AP targets OTUD1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human, mouse
文献引用反应性human, rat
免疫原

CatNo: Ag31996

Product name: Recombinant human OTUD1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 272-431 aa of

Sequence: SSAAEPVIVSRSDPRDEKLALYLAEVEKQDKYLRQRNKYRFHIIPDGNCLYRAVSKTVYGDQSLHRELREQTVHYIADHLDHFSPLIEGDVGEFIIAAAQDGAWAGYPELLAMGQMLNVNIHLTTGGRLESPTVSTMIHYLGPEDSLRPSIWLSWLSNGH

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 OTU domain containing 1
别名 OTU domain containing 1, OTDC1, DUBA7, DUBA 7
计算分子量51 kDa
观测分子量50-55 kDa
基因名称 OTUD1
Gene ID (NCBI) 220213
RRIDAB_3086190
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ5VV17
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

OTUD1, OTU domain-containing protein 1, is a deubiquitinating enzyme that specifically hydrolyzes 'Lys-63'-linked polyubiquitin to monoubiquitin (PMID: 23827681). OTUD1-mediated deubiquitination prevents activation of the ribosome quality control and subsequent dissociation of ribosomes and stimulates formation of polysomes and translation (PMID: 36445135). OTUD1 is a colon-specific deubiquitinase that expressed in normal colonic epithelium goblet cells (PMID: 36910614).

实验方案

Product Specific Protocols
WB protocol for OTUD1 antibody 29921-1-APDownload protocol
IHC protocol for OTUD1 antibody 29921-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IF

Nat Commun

Aggresome formation promotes ASK1/JNK signaling activation and stemness maintenance in ovarian cancer

Authors - Yurou Chen
View Article
humanIF

Mol Hum Reprod

OTUD1 inhibits endometriosis fibrosis by deubiquitinating MADH7

Authors - Xiangyu Chang
View Article

Int J Cardiol

BECN1 regulates FADD/RIPK1/Caspase-8 complex formation via RIPK1 ubiquitination by downregulating OTUD1 in MI/R induced myocyte apoptosis

Authors - Lu Hongquan
View Article
ratWB

Front Biosci (Landmark Ed)

VPO1 Promotes Programmed Necrosis of Cardiomyocytes in Rats with Chronic Heart Failure by Upregulating CYLD

Authors - Yinzhuang Zhang
View Article