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验证数据展示

  • WB analysis using 12066-1-AP (same clone as 12066-1-PBS)

    WB analysis using 12066-1-AP (same clone as 12066-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12066-1-AP (OTUB2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12066-1-PBS in a different storage buffer formulation.

  • IF Staining of U2OS using 12066-1-AP (same clone as 12066-1-PBS)

    IF Staining of U2OS using 12066-1-AP (same clone as 12066-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using OTUB2 antibody (12066-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 12066-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

OTUB2 Polyclonal antibody, PBS Only

OTUB2 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 12066-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

12066-1-PBS targets OTUB2 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag2703

Product name: Recombinant human OTUB2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-73 aa of BC009615

Sequence: MSETSFNLISEKCDILSILRDHPENRIYRRKIEELSKRFTAIRKTKGDGNCFYRALGYSYLESLLGKSREIFK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 OTU domain, ubiquitin aldehyde binding 2
别名
计算分子量 234 aa, 27 kDa
观测分子量27 kDa
GenBank蛋白编号BC009615
基因名称 OTUB2
Gene ID (NCBI) 78990
RRIDAB_3085388
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96DC9
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

OTUB2, also known as C14orf137, belongs to the OTU family. OTUB2 is a functional cysteine protease with deubiquitinase activity. OTUB2 mediates the deubiquitination of substrate proteins, and the targets of substrate proteins can be diversified. Therefore, OTUB2 has a wide range of biological roles, including protecting against virus-induced activation of interferon regulatory factor 3 (IRF3) and NF-κB, promoting beta cell survival in human pancreatic islets, and supporting the DNA repair pathway (PMID: 30662561, 35309903). OTUB2 is widely expressed, with high expression levels in the brain. OTUB2 has 2 isoforms with molecular weights of 9 kDa and 27 kDa.

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