验证数据展示
经过测试的应用
Positive WB detected in | mouse testis tissue, rat testis tissue |
Positive IF/ICC detected in | U2OS cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:500-1:2000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
12066-1-AP targets OTUB2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, IF/ICC, ELISA Application Description |
文献引用应用 | WB, IF |
经测试反应性 | human, mouse, rat |
文献引用反应性 | human |
免疫原 | OTUB2 fusion protein Ag2703 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | OTU domain, ubiquitin aldehyde binding 2 |
别名 | Otubain-2, OTU2, OTU domain-containing ubiquitin aldehyde-binding protein 2, OTB2, EC:3.4.19.12 |
计算分子量 | 234 aa, 27 kDa |
观测分子量 | 27 kDa |
GenBank蛋白编号 | BC009615 |
基因名称 | OTUB2 |
Gene ID (NCBI) | 78990 |
RRID | AB_3085388 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q96DC9 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
OTUB2, also known as C14orf137, belongs to the OTU family. OTUB2 is a functional cysteine protease with deubiquitinase activity. OTUB2 mediates the deubiquitination of substrate proteins, and the targets of substrate proteins can be diversified. Therefore, OTUB2 has a wide range of biological roles, including protecting against virus-induced activation of interferon regulatory factor 3 (IRF3) and NF-κB, promoting beta cell survival in human pancreatic islets, and supporting the DNA repair pathway (PMID: 30662561, 35309903). OTUB2 is widely expressed, with high expression levels in the brain. OTUB2 has 2 isoforms with molecular weights of 9 kDa and 27 kDa.
实验方案
Product Specific Protocols | |
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WB protocol for OTUB2 antibody 12066-1-AP | Download protocol |
IF protocol for OTUB2 antibody 12066-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |