• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

OTUB2 Polyclonal antibody

OTUB2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat No : 12066-1-AP

Print datasheet

Synonyms

C14orf137, Deubiquitinating enzyme OTUB2, OTB2, OTU2, OTUB2, Otubain 2, Ubiquitin thioesterase OTUB2

验证数据展示

  • WB analysis using 12066-1-AP

    WB analysis using 12066-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12066-1-AP (OTUB2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inmouse testis tissue, rat testis tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12066-1-AP targets OTUB2 in WB, ELISA applications and shows reactivity with mouse, rat samples.

Tested Applications WB, ELISA
Tested Reactivity mouse, rat
Immunogen OTUB2 fusion protein Ag2703 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name OTU domain, ubiquitin aldehyde binding 2
Synonyms C14orf137, Deubiquitinating enzyme OTUB2, OTB2, OTU2, OTUB2, Otubain 2, Ubiquitin thioesterase OTUB2
Calculated Molecular Weight 234 aa, 27 kDa
Observed Molecular Weight27 kDa
GenBank Accession NumberBC009615
Gene Symbol OTUB2
Gene ID (NCBI) 78990
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96DC9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

OTUB2, also known as C14orf137, belongs to the OTU family. OTUB2 is a functional cysteine protease with deubiquitinase activity. OTUB2 mediates the deubiquitination of substrate proteins, and the targets of substrate proteins can be diversified. Therefore, OTUB2 has a wide range of biological roles, including protecting against virus-induced activation of interferon regulatory factor 3 (IRF3) and NF-κB, promoting beta cell survival in human pancreatic islets, and supporting the DNA repair pathway (PMID: 30662561, 35309903). OTUB2 is widely expressed, with high expression levels in the brain. OTUB2 has 2 isoforms with molecular weights of 9 and 27 kDa.

实验方案

Product Specific Protocols
WB protocol for OTUB2 antibody 12066-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols