验证数据展示
经过测试的应用
| Positive WB detected in | mouse testis tissue, rat testis tissue |
| Positive IF/ICC detected in | U2OS cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12066-1-AP targets OTUB2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag2703 Product name: Recombinant human OTUB2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-73 aa of BC009615 Sequence: MSETSFNLISEKCDILSILRDHPENRIYRRKIEELSKRFTAIRKTKGDGNCFYRALGYSYLESLLGKSREIFK 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | OTU domain, ubiquitin aldehyde binding 2 |
| 别名 | Otubain-2, OTU2, OTU domain-containing ubiquitin aldehyde-binding protein 2, OTB2, EC:3.4.19.12 |
| 计算分子量 | 234 aa, 27 kDa |
| 观测分子量 | 27 kDa |
| GenBank蛋白编号 | BC009615 |
| 基因名称 | OTUB2 |
| Gene ID (NCBI) | 78990 |
| RRID | AB_3085388 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q96DC9 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
OTUB2, also known as C14orf137, belongs to the OTU family. OTUB2 is a functional cysteine protease with deubiquitinase activity. OTUB2 mediates the deubiquitination of substrate proteins, and the targets of substrate proteins can be diversified. Therefore, OTUB2 has a wide range of biological roles, including protecting against virus-induced activation of interferon regulatory factor 3 (IRF3) and NF-κB, promoting beta cell survival in human pancreatic islets, and supporting the DNA repair pathway (PMID: 30662561, 35309903). OTUB2 is widely expressed, with high expression levels in the brain. OTUB2 has 2 isoforms with molecular weights of 9 kDa and 27 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for OTUB2 antibody 12066-1-AP | Download protocol |
| WB protocol for OTUB2 antibody 12066-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Cell Biol Microtubule architecture connects AMOT stability to YAP/TAZ mechanotransduction and Hippo signalling | ||
Acta Pharm Sin B USP51/GRP78/ABCB1 axis confers chemoresistance through decreasing doxorubicin accumulation in triple-negative breast cancer cells | ||
Aging (Albany NY) The deubiquitinating enzymes-related signature predicts the prognosis and immunotherapy response in breast cancer
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