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验证数据展示

  • WB analysis using 10671-1-AP

    WB analysis using 10671-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10671-1-AP (OSTF1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 10671-1-AP

    IHC staining of human breast cancer using 10671-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 10671-1-AP (OSTF1 antibody) at dilution of 1:100 (under 25x lens).

  • IF Staining of A431 using 10671-1-AP

    IF Staining of A431 using 10671-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using OSTF1 antibody (10671-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

OSTF1 Polyclonal antibody

OSTF1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 10671-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

OSF, Osteoclast-stimulating factor 1, SH3P2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, C2C12 cells, HepG2 cells, U-937 cells
Positive IHC detected inhuman breast cancer tissue, human kidney tissue, human lung tissue, human placenta tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10671-1-AP targets OSTF1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human, mouse, rat
文献引用反应性human, rat
免疫原 OSTF1 fusion protein Ag1072 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 osteoclast stimulating factor 1
别名 OSF, Osteoclast-stimulating factor 1, SH3P2
计算分子量 28 kDa
观测分子量 28 kDa
GenBank蛋白编号BC007459
基因名称 OSTF1
Gene ID (NCBI) 26578
RRIDAB_2156717
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ92882
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

OSTF1 (Osteoclast-stimulating factor-1) is an intracellular protein produced by osteoclasts that indirectly induces osteoclast formation and bone resorption. It induces bone resorption, acting probably through a signaling cascade which results in the secretion of factor(s) enhancing osteoclast formation and activity

实验方案

Product Specific Protocols
WB protocol for OSTF1 antibody 10671-1-APDownload protocol
IHC protocol for OSTF1 antibody 10671-1-APDownload protocol
IF protocol for OSTF1 antibody 10671-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
rat,humanWB,IHC

Heliyon

OSTF1 knockdown mitigates IL-1β-induced chondrocyte injury via inhibiting the NF-κB signaling pathway

Authors - Bin Hu
View Article