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验证数据展示

  • WB analysis using 84555-2-RR (same clone as 84555-2-PBS)

    WB analysis using 84555-2-RR (same clone as 84555-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84555-2-RR (OSMR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84555-2-PBS in a different storage buffer formulation.

  • IHC staining of mouse cerebellum using 84555-2-RR (same clone as 84555-2-PBS)

    IHC staining of mouse cerebellum using 84555-2-RR (same clone as 84555-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 84555-2-RR (OSMR antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84555-2-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 84555-2-RR (same clone as 84555-2-PBS)

    FC experiment of HeLa using 84555-2-RR (same clone as 84555-2-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug OSMR Recombinant antibody (84555-2-RR, Clone:241865C8) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 84555-2-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84555-2-RR

    Affinity and Kinetic Characterization of 84555-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84555-2-RR against Human OSMR were performed. The affinity constant is 0.769 nM.

OSMR Recombinant monoclonal antibody, PBS Only

OSMR Uni-rAb® Recombinant Antibody for WB, IHC, FC (Intra), Indirect ELISA
Cat No. 84555-2-PBS

产品说明书

CloneNo. 241865C8

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, FC (Intra), Indirect ELISA

别名

241865C8, IL-31 receptor subunit beta, IL-31R subunit beta, IL-31RB, IL-31R-beta

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

84555-2-PBS targets OSMR in WB, IHC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag1426

Product name: Recombinant human OSMR protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-342 aa of BC010943

Sequence: MALFAVFQTTFFLTLLSLRTYQSEVLAERLPLTPVSLKVSTNSTRQSLHLQWTVHNLPYHQELKMVFQIQISRIETSNVIWVGNYSTTVKWNQVLHWSWESELPLECATHFVRIKSLVDDAKFPEPNFWSNWSSWEEVSVQDSTGQDILFVFPKDKLVEEGTNVTICYVSRNIQNNVSCYLEGKQIHGEQLDPHVTAFNLNSVPFIRNKGTNIYCEASQGNVSEGMKGIVLFVSKVLEEPKDFSCETEDFKTLHCTWDPGTDTALGWSKQPSQSYTLFESFSGEKKLCTHKNWCNWQITQDSQETYNFTLIAENYLRKRSVNILFNLTHRGETRVVTAHRGH

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 oncostatin M receptor
别名 241865C8, IL-31 receptor subunit beta, IL-31R subunit beta, IL-31RB, IL-31R-beta
计算分子量 110 kDa
观测分子量~180 kDa
GenBank蛋白编号BC010943
基因名称 OSMR
Gene ID (NCBI) 9180
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ99650
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Oncostatin M (OSM) is a member of interleukin-6 family cytokines that modulates proliferation and differentiation in numerous cells (PMID: 22982441). OSM signals via a receptor complex comprising gp130 and the OSMR (also called OSMRβ) (PMID: 8999038). OSMR is a single-pass membrane protein that belongs to the type I cytokine receptor family. OSMR mRNA is expressed at relatively high levels in all neural cells and fibroblast, epithelial, and various tumor cell lines (PMID: 8999038). It has also been shown that OSMR associates with IL31RA to form the heterodimeric IL31 receptor (PMID: 15184896). OMSR has a calculated molecular weight of 110 kDa, with an apparent molecular weight of 150-180 kDa (PMID: 8999038; 17028186; 39266541; 34644107). A 70 kDa soluble form can be detected by western blot (PMID: 17028186).

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