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验证数据展示

  • WB analysis of HepG2 using 15491-1-AP

    WB analysis of HepG2 using 15491-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 15491-1-AP (OSBPL10 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa cells using 15491-1-AP

    IP experiment of HeLa cells using 15491-1-AP

    IP result of anti-OSBPL10 (15491-1-AP for IP and Detection).

  • IHC staining of human colon cancer using 15491-1-AP

    IHC staining of human colon cancer using 15491-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 15491-1-AP (OSBPL10 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human colon cancer using 15491-1-AP

    IHC staining of human colon cancer using 15491-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 15491-1-AP (OSBPL10 antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HepG2 using 15491-1-AP

    IF Staining of HepG2 using 15491-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using OSBPL10 antibody (15491-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

OSBPL10 Polyclonal antibody

OSBPL10 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 15491-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, ELISA and More (1)

别名

OSBP-related protein 10, OSBP9, OSBP related protein 10, ORP-10, ORP10

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15491-1-AP targets OSBPL10 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 OSBPL10 fusion protein Ag7861 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 oxysterol binding protein-like 10
别名 OSBP-related protein 10, OSBP9, OSBP related protein 10, ORP-10, ORP10
计算分子量 84 kDa
观测分子量 75-80 kDa
GenBank蛋白编号BC003168
基因名称 OSBPL10
Gene ID (NCBI) 114884
RRIDAB_2158356
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BXB5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

OSBPL10, also known as ORP10, is a member of the oxysterol-binding protein family. OSBPL10 localizes on microtubules and acts as a regulator of cellular lipid biosyntheses and apolipoprotein B-100. Genetic variation in OSBPL10 is associated with dyslipidmias and peripheral artery disease.

实验方案

Product Specific Protocols
WB protocol for OSBPL10 antibody 15491-1-APDownload protocol
IHC protocol for OSBPL10 antibody 15491-1-APDownload protocol
IF protocol for OSBPL10 antibody 15491-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Biochim Biophys Acta

ORP10, a cholesterol binding protein associated with microtubules, regulates apolipoprotein B-100 secretion.

Authors - Nissil? Eija E
View Article
humanIF,WB

Nat Commun

Regulation of cellular cholesterol distribution via non-vesicular lipid transport at ER-Golgi contact sites

Authors - Tomoki Naito
View Article